In vitro oxidation of LDL by ozone

•We show that ozone is capable of oxidizing low-density lipoprotein (LDL).•LPO, TBARS, REM, and oxidation-specific epitopes increase upon LDL oxidation by ozone.•Ozonization of LDL may be a novel pathway which supports atherogenesis. Recent studies suggest that ozone is present in atherosclerotic le...

Full description

Saved in:
Bibliographic Details
Published in:Chemistry and physics of lipids 2014-10, Vol.183, p.18-21
Main Authors: Hörl, Gerd, Ledinski, Gerhard, Kager, Gerd, Hallström, Seth, Tafeit, Erwin, Koestenberger, Martin, Jürgens, Günther, Cvirn, Gerhard
Format: Article
Language:English
Subjects:
Atherosclerosis
Humans
Kinetics
Lipid hydroperoxides
Lipoproteins, LDL - chemistry
Low-density lipoprotein
Male
Oxidation-Reduction
Oxidation-specific immune epitopes
Ozone
Ozone - chemistry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:•We show that ozone is capable of oxidizing low-density lipoprotein (LDL).•LPO, TBARS, REM, and oxidation-specific epitopes increase upon LDL oxidation by ozone.•Ozonization of LDL may be a novel pathway which supports atherogenesis. Recent studies suggest that ozone is present in atherosclerotic lesions. Since these lesions are characterized by a dramatic accumulation of low-density lipoprotein (LDL), we aimed to investigate whether ozone is capable of oxidizing LDL, thereby rendering this lipoprotein atherogenic. Lipid hydroperoxide (LPO) concentrations and thiobarbituric acid reactive substances (TBARS) were measured to assess the oxidative status of the lipid part of LDL. Relative electrophoretic mobility (REM) and oxidation-specific immune epitopes were measured to assess the oxidative status of the protein part (apoB) of the LDL particle. Ozone turned out to be a potent oxidant of LDL. LPO concentrations, TBARS, REM, and oxidation-specific immune epitopes significantly increased upon ozonization. Our results suggest that ozonization of LDL may be a novel pathway which supports atherogenesis. Ozone is capable of oxidizing the lipid part of LDL, followed by immediate oxidation of the protein part of LDL, rendering the lipoprotein atherogenic.
ISSN:0009-3084
1873-2941
DOI:10.1016/j.chemphyslip.2014.05.002