Transcriptional response machineries of Bacillus subtilis conducive to plant growth promotion

• Published in: Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2014-09, Vol.78 (9), p.1471-1484
• Main Author: Hirooka, Kazutake
• Format: Article
• Language: English
• Subjects: Bacillus subtilis; Bacillus subtilis - metabolism; Bacillus subtilis - pathogenicity; Bacterial Proteins - genetics; copper; Fabaceae - genetics; Fabaceae - growth & development; Fabaceae - microbiology; flavonoid; Flavonoids - chemistry; Flavonoids - metabolism; Gene Expression Regulation, Bacterial; plant growth-promoting rhizobacteria; Plant Roots - genetics; Plant Roots - growth & development; Plant Roots - microbiology; Plants - microbiology; Promoter Regions, Genetic; Protein Binding - genetics; Rhizosphere; Symbiosis - genetics; Transcription Factors - biosynthesis; Transcription Factors - genetics; transcriptional regulation
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1080/09168451.2014.943689

Bacillus subtilis collectively inhabits the rhizosphere, where it contributes to the promotion of plant growth, although it does not have a direct symbiotic relationship to plants as observed in the case of rhizobia between leguminous plants. As rhizobia sense the flavonoids released from their host roots through the NodD transcriptional factor, which triggers transcription of the nod genes involved in the symbiotic processes, we supposed that B. subtilis utilizes certain flavonoids as signaling molecules to perceive and adapt to the rhizospheric environment that it is in. Our approaches to identify the flavonoid-responsive transcriptional regulatory system from B. subtilis resulted in the findings that three transcriptional factors (LmrA/QdoR, YetL, and Fur) are responsive to flavonoids, with the modes of action being different from each other. We also revealed a unique regulatory system by two transcriptional factors, YcnK and CsoR, for copper homeostasis in B. subtilis. In this review, we summarize the molecular mechanisms of these regulatory systems with the relevant information and discuss their physiological significances in the mutually beneficial interaction between B. subtilis and plants, considering the possibility of their application for plant cultivation. Bacillus subtilis utilizes flavonoids as signaling molecules to perceive and adapt to the rhizospheric environment. It contributes to the plant growth promotion by enhancing the iron uptake from roots and by suppressing the propagation of phytopathogens.