The protective role of thymoquinone in the prevention of gentamicin ototoxicity

Abstract Objective To investigate the potential protective effect of thymoquinone in gentamicin-induced ototoxicity through auditory brain stem responses (ABR) testing and histomorphological evaluation of the cochlea. Methods This study was conducted on 48 adult female Sprague–Dawley rats that were...

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Bibliographic Details
Published in:American journal of otolaryngology 2014-09, Vol.35 (5), p.603-609
Main Authors: Sagit, Mustafa, MD, Korkmaz, Ferhat, MD, Gürgen, Seren Gulsen, MD, Kaya, Mesut, MD, Akcadag, Alper, PhD, Ozcan, Ibrahim, MD
Format: Article
Language:English
Subjects:
Animals
Apoptosis
Benzoquinones - pharmacology
Cochlea - drug effects
Drug dosages
Ears & hearing
Female
Gentamicins - toxicity
Hearing loss
Hearing Loss - chemically induced
Hearing Loss - prevention & control
In Situ Nick-End Labeling
Laboratories
Liver
Otolaryngology
Random Allocation
Rats
Rats, Sprague-Dawley
Rodents
Studies
Surgery
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Summary:Abstract Objective To investigate the potential protective effect of thymoquinone in gentamicin-induced ototoxicity through auditory brain stem responses (ABR) testing and histomorphological evaluation of the cochlea. Methods This study was conducted on 48 adult female Sprague–Dawley rats that were randomized into 4 groups. Group 1 received intraperitoneal gentamicin; group 2 received intraperitoneal gentamicin plus corn oil solution; group 3 received intraperitoneal thymoquinone; and group 4 received intraperitoneal gentamicin plus thymoquinone. All groups received the drugs (once daily) in the above-mentioned protocols over 15 days. After conducting repeated ABR measurements, the rats were sacrificed, and their cochleae were isolated. Results ABR thresholds were preserved in the gentamicin plus thymoquinone group when compared with the group receiving gentamicin alone. There were fewer TUNEL-positive cells and caspase-3 and caspase-9 expressions were weaker in the inner and outer hairy cells of the organ of Corti in the gentamicin plus thymoquinone group compared with the group receiving gentamicin alone. Conclusion The ABR values and number of apoptotic cells did not significantly increase in the group receiving gentamicin plus thymoquinone when compared to the group receiving gentamicin alone. Again, the cochlear histomorphological findings were supportive of the auditory findings. In light of these findings, we conclude that gentamicin-induced ototoxicity may be prevented by thymoquinone use in rats.
ISSN:0196-0709
1532-818X
DOI:10.1016/j.amjoto.2014.07.002