Longitudinal evaluation of fluoride levels in nails of 18-30-month-old children that were using toothpastes with 500 and 1100 μg F/g

• Published in: Community dentistry and oral epidemiology 2014-10, Vol.42 (5), p.412-419
• Main Authors: Amaral, Jackeline G., Freire, Isabelle R., Valle-Neto, Eduardo F. R., Cunha, Robson F., Martinhon, Cleide C. R., Delbem, Alberto C. B.
• Format: Article
• Language: English
• Subjects: Cariostatic Agents - chemistry; Child, Preschool; Dentistry; Female; fluoride; Fluorides - analysis; Humans; Infant; Longitudinal Studies; Male; nails; Nails - chemistry; toothpaste; Toothpastes - chemistry
• ISSN: 0301-5661; 1600-0528
• DOI: 10.1111/cdoe.12103

Objectives This study aimed to evaluate the fluoride concentration in the fingernails and toenails of children aged 18–30 months during use of fluoride‐containing toothpastes supplemented with calcium glycerophosphate (CaGP) or sodium trimetaphosphate (TMP). Methods According to the toothpaste used, children (n = 56) were randomly assigned into three groups: 500 μg F/g with 1% TMP, 500 μg F/g with 0.25% CaGP, and 1100 μg F/g. Fingernails and toenails were collected monthly over a period of 330 days, from the beginning of toothpaste use. Fluoride concentration in the water consumed by the volunteers and fluoride intake from diet and toothpaste were also determined. Fluoride analyses were performed with the electrode after hexamethyldisiloxane‐facilitated diffusion or by the direct method, according to the samples. Data passed normality and homoscedasticity tests and were analyzed by 2‐way analysis of variance (anova) and 1‐way anova followed by Student‐Newman‐Keuls test (P