Cross-species transferability of eastern white pine (Pinus strobus) nuclear microsatellite markers to five Mexican white pines

We examined cross-species transferability and usefulness of six nuclear microsatellite markers developed in consubgeneric eastern white pine (Pinus strobus) with regard to ecologically and commercially important Mexican white pine species of conservation genetics concern: Pinus chiapensis (Mart.) An...

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Bibliographic Details
Published in:Genetics and molecular research 2014-09, Vol.13 (3), p.7571-7576
Main Authors: Villalobos-Arámbula, A R, Pérez de la Rosa, J A, Arias, A, Rajora, O P
Format: Article
Language:English
Subjects:
Crosses, Genetic
Genetic Loci
Genetics, Population
Mexico
Microsatellite Repeats
Pinus - genetics
Polymorphism, Genetic
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Summary:We examined cross-species transferability and usefulness of six nuclear microsatellite markers developed in consubgeneric eastern white pine (Pinus strobus) with regard to ecologically and commercially important Mexican white pine species of conservation genetics concern: Pinus chiapensis (Mart.) Andresen, P. flexilis James, P. strobiformis Engelm., P. ayacahuite Ehrenb. Ex Schltdl, and P. ayacahuite var. veitchii (Roezl) G.R. Shaw. Four to six microsatellite loci were found to be polymorphic in different species, with moderate to high informativeness in a relatively small number of samples (PIC/HE=0.25-0.93). This successful transfer sidesteps the time- and resource-consuming development of species-specific microsatellite markers, and will facilitate population and conservation genetic studies and genetic resource management of the less studied Mexican white pines.
ISSN:1676-5680
DOI:10.4238/2014.September.12.24