Early expression of a hatching enzyme gene in masu salmon (Oncorhynchus masou) embryos

Hatching enzymes of fishes are developmentally regulated proteases secreted from the hatching gland before hatching. We purified the hatching enzyme of masu salmon (Oncorhynchus masou) to one protein, determined the N-terminal 19 amino acid sequence, and synthesized DNA in accordance with this amino...

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Bibliographic Details
Published in:Canadian journal of fisheries and aquatic sciences 1996, Vol.53 (3), p.509-512
Main Authors: Araki, K, Fujikawa, N, Nakayama, I, Nagoya, H, Onozato, H
Format: Article
Language:English
Subjects:
Animal aquaculture
Animal productions
Biological and medical sciences
Freshwater
Fundamental and applied biological sciences. Psychology
Marine
Oncorhynchus masou
Pisciculture
Vertebrate aquaculture
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Summary:Hatching enzymes of fishes are developmentally regulated proteases secreted from the hatching gland before hatching. We purified the hatching enzyme of masu salmon (Oncorhynchus masou) to one protein, determined the N-terminal 19 amino acid sequence, and synthesized DNA in accordance with this amino acid sequence. The developmental stage expressing the hatching enzyme gene was determined with Northern hybridization using this synthesized DNA as a probe. The hatching enzyme gene of masu salmon was expressed in the stage from the 20th to 28th days. Masu salmon embryos usually hatch out at the 40th day stage. Therefore, the hatching enzyme gene is expressed at an early stage before hatching.
ISSN:0706-652X
1205-7533
DOI:10.1139/f95-210