Complete Nucleotide Sequences of the Domestic Cat (Felis catus) Mitochondrial Genome and a Transposed mtDNA Tandem Repeat (Numt) in the Nuclear Genome

• Published in: Genomics (San Diego, Calif.) Calif.), 1996-04, Vol.33 (2), p.229-246
• Main Authors: Lopez, Jose V., Cevario, Stanley, O'Brien, Stephen J.
• Format: Article
• Language: English
• Subjects: ADN; Amino Acid Sequence; AMINO ACID SEQUENCES; Animals; ARN DE TRANSFERENCIA; ARN DE TRANSFERT; ARN RIBOSOMAL; ARN RIBOSOMIAL; base composition; Base Sequence; Biological and medical sciences; CATS; Cats - genetics; Cell Nucleus; CHAT; CHEMICAL COMPOSITION; CODE GENETIQUE; CODIGO GENETICO; codon usage; COMPOSICION QUIMICA; COMPOSITION CHIMIQUE; DELETIONS; DNA; DNA Transposable Elements; DNA, Mitochondrial; Felis catus; FILOGENIA; Fundamental and applied biological sciences. Psychology; GATO; genbank/u20753; genbank/u20754; GENE; GENES; Genes. Genome; GENETIC CODE; GENETICA; GENETICS; GENETIQUE; GENOMAS; GENOME; GENOMES; Humans; insertions; MITOCHONDRIA; MITOCHONDRIE; MITOCONDRIA; Molecular and cellular biology; MOLECULAR CONFORMATION; Molecular genetics; Molecular Sequence Data; MUTACION; MUTATION; NOYAU CELLULAIRE; NUCLEI; Nucleic Acid Conformation; NUCLEO; NUCLEOCYTOPLASMIC INTERACTION; NUCLEOTIDE SEQUENCE; NUCLEUS; open reading frames; PHYLOGENIE; PHYLOGENY; REPETITIVE DNA; Repetitive Sequences, Nucleic Acid; RESTRICTION MAPPING; RIBOSOMAL RNA; RIBOSOMAS; RIBOSOME; RIBOSOMES; SECUENCIA NUCLEICA; SEQUENCE NUCLEIQUE; STRUCTURAL GENES; substitutions; TRANSFER; TRANSFER RNA
• ISSN: 0888-7543; 1089-8646
• DOI: 10.1006/geno.1996.0188

The complete 17,009-bp mitochondrial genome of the domestic cat,Felis catus,has been sequenced and conforms largely to the typical organization of previously characterized mammalian mtDNAs. Codon usage and base composition also followed canonical vertebrate patterns, except for an unusual ATC (non-AUG) codon initiating the NADH dehydrogenase subunit 2 (ND2) gene. Two distinct repetitive motifs at opposite ends of the control region contribute to the relatively large size (1559 bp) of this carnivore mtDNA. Alignment of the feline mtDNA genome to a homologous 7946-bp nuclear mtDNA tandem repeat DNA sequence in the cat,Numt,indicates simple repeat motifs associated with insertion/deletion mutations. Overall DNA sequence divergence betweenNumtand cytoplasmic mtDNA sequence was only 5.1%. Substitutions predominate at the third codon position of homologous feline protein genes. Phylogenetic analysis of mitochondrial gene sequences confirms the recent transfer of the cytoplasmic mtDNA sequences to the domestic cat nucleus and recapitulates evolutionary relationships between mammal species.