Regulation of Mg, K-ATPase activity in guard cells of Commelina communis by phytochrome and ABA

The microsomal fraction obtained from guard cell protoplasts was assayed for ATPase activity at pH 6.5. Triton X-100 did not affect this stimulation of activity of ATPase by K+ up to 5 mM, but the detergent abolished the stimulatory effect of K+ at higher concentrations. The ATPase activity was inhi...

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Bibliographic Details
Published in:Plant and cell physiology 1989-10, Vol.30 (7), p.945-949
Main Authors: Nejidat, A. (Ben Gurion Univ., Beer Sheava (Israel)), Itai, C, Bejerano, N.R
Format: Article
Language:English
Subjects:
ABA
abscisic acid
ACIDE ABSCISSIQUE
ACIDO ABSCISICO
ADENOSINA TRIFOSFATASA
ADENOSINE TRIPHOSPHATASE
Biological and medical sciences
DCCD
Enzymes
FARINOSAE
FITOCROMA
Fundamental and applied biological sciences. Psychology
Metabolism
Mg-ATPase
Mg/K-transporting ATPase
PHYTOCHROME
PHYTOCHROMES
Plant physiology and development
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Summary:The microsomal fraction obtained from guard cell protoplasts was assayed for ATPase activity at pH 6.5. Triton X-100 did not affect this stimulation of activity of ATPase by K+ up to 5 mM, but the detergent abolished the stimulatory effect of K+ at higher concentrations. The ATPase activity was inhibited by N,N-dicyclohexylcarbodiimide and ABA. Irradiation with red light enhanced the ATPase activity more than did irradiation with far-red light. ABA and irradiation with red or far-red light were effective only in the presence of K+. These results suggest the possibility that the ATPase activity is modulated only indirectly by light and ABA.
ISSN:0032-0781
1471-9053
1471-9053
0032-0781
DOI:10.1093/oxfordjournals.pcp.a077838