nucleic acid sequence-based amplification (NASBA) system for Listeria monocytogenes and simple method for detection of amplimers

A NASBA system amplifying specific sequences of the Listeria monocytogenes hlyA gene and an immunoenzymatic assay for the detection of amplimers was developed. The immunoenzymatic assay utilized a simple microtiter plate format and an anti-RNA:DNA hybrid antibody for the detection of NASBA product (...

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Bibliographic Details
Published in:Biotechnology techniques 1996, Vol.10 (3), p.189-194
Main Authors: Blais, B.W, Turner, G, Sooknanan, R, Malek, L.T, Phillippe, L.M
Format: Article
Language:English
Subjects:
antibodies
Bacteriological methods and techniques used in bacteriology
Bacteriology
biochemical techniques
Biological and medical sciences
detection
DNA
DNA probes
Food industries
Food microbiology
Fundamental and applied biological sciences. Psychology
hemolysins
hly gene
immobilization
immunoenzyme techniques
Listeria monocytogenes
Microbiology
RNA
rna dna hybrids
structural genes
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Summary:A NASBA system amplifying specific sequences of the Listeria monocytogenes hlyA gene and an immunoenzymatic assay for the detection of amplimers was developed. The immunoenzymatic assay utilized a simple microtiter plate format and an anti-RNA:DNA hybrid antibody for the detection of NASBA product (predominantly RNA) hybridized to an immobilized DNA probe. This highly sensitive isothermal amplification and detection system was reactive with genomic DNA from various L. monocytogenes isolates but not with other Listeria or non-Listeria species.
ISSN:0951-208X
1573-6784
DOI:10.1007/BF00158944