A New Dual-Channel Optical Signal Probe for Cu2+ Detection Based on Morin and Boric Acid

In this work we utilized the common analytical reagent morin to develop a new a dual-channel, cost-effective, and sensitive method for determination of Cu2+. It is found that morin is only weakly fluorescent by itself, but forms highly fluorescent complexes with boric acid. Moreover, the fluorescenc...

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Bibliographic Details
Published in:Applied spectroscopy 2014-10, Vol.68 (10), p.1148-1153
Main Authors: Wang, Peng, Yuan, Bin Fang, Li, Nian Bing, Luo, Hong Qun
Format: Article
Language:English
Subjects:
Boric Acids - chemistry
Copper - analysis
Copper - chemistry
Flavonoids - chemistry
Limit of Detection
Reproducibility of Results
Spectrometry, Fluorescence - methods
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Summary:In this work we utilized the common analytical reagent morin to develop a new a dual-channel, cost-effective, and sensitive method for determination of Cu2+. It is found that morin is only weakly fluorescent by itself, but forms highly fluorescent complexes with boric acid. Moreover, the fluorescence of complexes of morin with boric acid is quenched linearly by Cu2+ in a certain concentration range. Under optimum conditions, the fluorescence quenching efficiency was linearly proportional to the concentration of cupric ions in the range of 0.5-25 μM with high sensitivity, and the detection limit for Cu2+ was 0.38 μM. The linear range was 1-25 μM determined by spectrophotometry, and the detection limit for cupric ions was 0.8 μM. Furthermore, the mechanism of sensitive fluorescence quenching response of morin to Cu2+ is discussed.
ISSN:0003-7028
1943-3530
DOI:10.1366/13-07233