Flavobacterium ahnfeltiae sp. nov., a new marine polysaccharide-degrading bacterium isolated from a Pacific red alga

• Published in: Archives of microbiology 2014-10, Vol.196 (10), p.745-752
• Main Authors: Nedashkovskaya, Olga I, Balabanova, Larissa A, Zhukova, Natalia V, Kim, So-Jeong, Bakunina, Irina Y, Rhee, Sung-Keun
• Format: Article
• Language: English
• Subjects: Ahnfeltia; Algae; bacteria; Bacterial Typing Techniques; Base Composition; Biochemistry; Biomedical and Life Sciences; Biotechnology; Cell Biology; DNA; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; Ecology; Fatty acids; Fatty Acids - analysis; Flavobacterium; Flavobacterium - classification; Flavobacterium - genetics; Flavobacterium - isolation & purification; Flavobacterium - metabolism; Genes, rRNA; Life Sciences; Lipids; Lipids - analysis; Microbial Ecology; Microbiology; new species; nucleotide sequences; Phaeophycophyta; phosphatidylethanolamines; Phylogenetics; Phylogeny; polysaccharides; Polysaccharides - metabolism; Rhodophyta - microbiology; ribosomal RNA; RNA, Ribosomal, 16S - genetics; Saccharides; Sequence Analysis, DNA; sequence homology; Short Communication; Taxonomy
• ISSN: 0302-8933; 1432-072X
• DOI: 10.1007/s00203-014-1010-2

A Gram-negative, aerobic, rod-shaped, motile by gliding and yellow-pigmented bacterium, designated strain 10Alg 130ᵀ, that displayed the ability to destroy polysaccharides of red and brown algae, was isolated from the red alga Ahnfeltia tobuchiensis. The phylogenetic analysis based on 16S rRNA gene sequence placed the novel strain within the genus Flavobacterium, the type genus of the family Flavobacteriaceae, the phylum Bacteroidetes, with sequence similarities of 96.2 and 95.7 % to Flavobacterium jumunjiense KCTC 23618ᵀ and Flavobacterium ponti CCUG 58402ᵀ, and 95.3–92.5 % to other recognized Flavobacterium species. The prevalent fatty acids of strain 10Alg 130ᵀ were iso-C₁₅:₀, iso-C₁₅:₀ 3-OH, iso-C₁₇:₀ 3-OH, C₁₅:₀ and iso-C₁₇:₁ω9c. The polar lipid profile consisted of phosphatidylethanolamine, two unknown aminolipids and three unknown lipids. The DNA G+C content of the type strain was 34.3 mol%. The new isolate and the type strains of recognized species of the genus Flavobacterium could strongly be distinguished by a number of phenotypic characteristics. A combination of the genotypic and phenotypic data showed that the algal isolate represents a novel species of the genus Flavobacterium, for which the name Flavobacterium ahnfeltiae sp. nov. is proposed. The type strain is 10Alg 130ᵀ (=KCTC 32467ᵀ = KMM 6686ᵀ).