Immunoelectron microscopy of Bacillus subtilis cells secreting human interferon alpha sub(1) or staphylokinase

Post-embedding labelling techniques with colloidal gold-IgG or -protein A complexes were used to determine the subcellular location of IFN alpha sub(1) and staphylokinase secreted from Bacillus subtilis) GB500 cells. Both proteins were present in the cytoplasma and the cell envelope pointing to a po...

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Bibliographic Details
Published in:FEMS microbiology letters 1989-01, Vol.65 (3), p.327-332
Main Authors: Wagner, B, Wagner, M, Wollweber, L, Behnke, D
Format: Article
Language:English
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Summary:Post-embedding labelling techniques with colloidal gold-IgG or -protein A complexes were used to determine the subcellular location of IFN alpha sub(1) and staphylokinase secreted from Bacillus subtilis) GB500 cells. Both proteins were present in the cytoplasma and the cell envelope pointing to a posttranslational mode of translocation across the cytoplasmic membrane. 5- to 10-fold higher concentrations of gold particles per 0.1 mu m super(2) were found on the cell envelope and clustering was observed suggesting preferential regions for secretion sites. Several control experiments ensured the specificity of the labelling data.
ISSN:0378-1097