Immunoinformatics study on highly expressed Mycobacterium tuberculosis genes during infection

Summary The most important targets for vaccine development are the proteins that are highly expressed by the microorganisms during infection in-vivo . A number of Mycobacterium tuberculosis (Mtb) proteins are also reported to be expressed in-vivo at different phases of infection. In the present stud...

Full description

Saved in:
Bibliographic Details
Published in:Tuberculosis (Edinburgh, Scotland) Scotland), 2014-09, Vol.94 (5), p.475-481
Main Authors: Nguyen Thi, Le Thuy, Sarmiento, Maria Elena, Calero, Romel, Camacho, Frank, Reyes, Fatima, Hossain, Md Murad, Gonzalez, Gustavo Sierra, Norazmi, Mohd Nor, Acosta, Armando
Format: Article
Language:English
Subjects:
Animals
Bacterial Proteins - genetics
Bacterial Proteins - immunology
Computational Biology
Databases, Genetic
Drug Design
Epitopes, B-Lymphocyte - genetics
Epitopes, T-Lymphocyte - genetics
Gene Expression Regulation, Bacterial
Humans
Infectious Disease
Mycobacterium tuberculosis - genetics
Mycobacterium tuberculosis - immunology
Mycobacterium tuberculosis - pathogenicity
Neural Networks (Computer)
Pulmonary/Respiratory
Tuberculosis - genetics
Tuberculosis - immunology
Tuberculosis - microbiology
Tuberculosis - prevention & control
Tuberculosis Vaccines - immunology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Summary The most important targets for vaccine development are the proteins that are highly expressed by the microorganisms during infection in-vivo . A number of Mycobacterium tuberculosis (Mtb) proteins are also reported to be expressed in-vivo at different phases of infection. In the present study, we analyzed multiple published databases of gene expression profiles of Mtb in-vivo at different phases of infection in animals and humans and selected 38 proteins that are highly expressed in the active, latent and reactivation phases. We predicted T- and B-cell epitopes from the selected proteins using HLAPred for T-cell epitope prediction and BCEPred combined with ABCPred for B-cell epitope prediction. For each selected proteins, regions containing both T- and B-cell epitopes were identified which might be considered as important candidates for vaccine design against tuberculosis.
ISSN:1472-9792
1873-281X
DOI:10.1016/j.tube.2014.06.004