The new sulfated O-specific polysaccharide from marine bacterium Cobetia pacifica KMM 3878, containing 3,4-O-[(S)-1-carboxyethylidene]-d-galactose and 2,3-O-disulfate-d-galactose

• Published in: Carbohydrate research 2014-10, Vol.397, p.46-51
• Main Authors: Kokoulin, Maxim S., Kalinovsky, Anatoliy I., Komandrova, Nadezhda A., Tomshich, Svetlana V., Romanenko, Lyudmila A., Vaskovsky, Victor E.
• Format: Article
• Language: English
• Subjects: 2,3-O-disulfate-d-galactose; 3,4-O-[(S)-1-carboxyethylidene]-d-galactose; Aquatic Organisms; Carbohydrate Sequence; Cobetia pacifica; Halomonadaceae - chemistry; Magnetic Resonance Spectroscopy; Marine bacterium; Molecular Sequence Data; O Antigens - chemistry; O Antigens - isolation & purification; O-specific polysaccharide; Sulfate
• ISSN: 0008-6215; 1873-426X
• DOI: 10.1016/j.carres.2014.06.006

[Display omitted] →4)-β-d-Gal2,3R-(1→6)-β-d-Gal3,4(S-Pyr)-(1→6)-β-d-Gal-(1→ Where R is –SO3H. •Structure of the O-polysaccharide of C. pacifica KMM 3878 was established.•The O-polysaccharide is built up of sulfated trisaccharide repeating units.•The simultaneous presence of d-Gal3,4(S-Pyr) and d-Gal2,3SO3− was found in OPS. The O-specific polysaccharide was isolated from the lipopolysaccharide of Cobetia pacifica KMM 3878 and studied by chemical methods along with 1H and 13C NMR spectroscopy, including, 1D TOCSY and 2D 1H, 1H COSY, 1H, 13C HSQC, 1H, 1H ROESY, 1H, 13C HMBC and 1H, 13C H2BC experiments. The following new structure of the sulfated O-polysaccharide from C. pacifica KMM 3878 containing 3,4-O-[(S)-1-carboxyethylidene]-d-galactose and 2,3-O-disulfate-d-galactose was established: →4)-β-d-Gal2,3R-(1→6)-β-d-Gal3,4(S-Pyr)-(1→6)-β-d-Gal-(1→ Where R is –SO3H.