Short communication: Antimicrobial susceptibility profiling and genotyping of Staphylococcus aureus isolates from bovine mastitis in Poland

• Published in: Journal of dairy science 2014-10, Vol.97 (10), p.6122-6128
• Main Authors: Jagielski, T., Puacz, E., Lisowski, A., Siedlecki, P., Dudziak, W., Międzobrodzki, J., Krukowski, H.
• Format: Article
• Language: English
• Subjects: Animals; Anti-Bacterial Agents - therapeutic use; Anti-Infective Agents - therapeutic use; beta-Lactamases; beta-Lactams; bovine mastitis; Cattle; DNA, Bacterial - analysis; DNA, Bacterial - genetics; drug resistance; Female; Genotype; genotyping; Mastitis, Bovine - drug therapy; Mastitis, Bovine - epidemiology; Mastitis, Bovine - microbiology; Microbial Sensitivity Tests - veterinary; Penicillin Resistance - genetics; Poland; Staphylococcal Infections - drug therapy; Staphylococcal Infections - veterinary; Staphylococcus aureus; Staphylococcus aureus - drug effects; Staphylococcus aureus - genetics; Staphylococcus aureus - isolation & purification
• ISSN: 0022-0302; 1525-3198
• DOI: 10.3168/jds.2014-8321

Staphylococcus aureus is the predominant causative agent of bovine mastitis, a disease that remains a major economic burden for the dairy industry worldwide. In this study, the antimicrobial resistance patterns and the genetic composition of 80 S. aureus mastitis isolates collected from 14 dairy farms in Eastern Poland were determined. Of the 10 antimicrobial agents evaluated, only testing for penicillin G produced drug resistance. As 41% of the S. aureus isolates were penicillin resistant, this drug along with other β-lactamase-sensitive β-lactams, should rather not be considered for the treatment of bovine mastitis caused by S. aureus. Upon genotyping, with a triplex PCR method, a total of 11 distinct PCR types were produced. The population structure of S. aureus isolates was highly clonal, with 1 predominant genotype circulating on each farm. The observed similarities in the genotype composition of S. aureus populations from geographically distant farms underscore the significance of interfarm transmission of S. aureus in Poland. This, in turn, argues for the establishment of a nationwide surveillance program for bovine mastitis due to this pathogen.