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BMQ_0737 encodes a novel protein crucial to the integrity of the outermost layers of Bacillus megaterium QM B1551 spores

Abstract Bioinformatic and electron microscopy analyses indicate that the composition of the B. megateriumQM B1551 spore coat is likely to differ substantially from other Bacillus species. We report here on the identification and characterisation of novel B. megaterium proteins that appear to be abu...

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Bibliographic Details
Published in:FEMS microbiology letters 2014-09, Vol.358 (2), p.162-169
Main Authors: Manetsberger, Julia, Hall, Elizabeth A.H., Christie, Graham
Format: Article
Language:English
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Summary:Abstract Bioinformatic and electron microscopy analyses indicate that the composition of the B. megateriumQM B1551 spore coat is likely to differ substantially from other Bacillus species. We report here on the identification and characterisation of novel B. megaterium proteins that appear to be abundant in the spore coat. All three proteins, encoded by loci BMQ_0737, BMQ_3035 and BMQ_4051, were identified by proteomic analysis of alkaline detergent extracts from mature spores. Putative spore coat proteins were characterised by transcriptional, reporter-fusion and mutagenesis analyses supported by fluorescence and transmission electron microscopy. These analyses revealed that BMQ_0737 is a novel morphogenetic protein that is required for the correct assembly of the B. megaterium outer spore coat and exosporium, both of which are structurally compromised or missing in BMQ_0737 null mutant spores. The first proteomic analysis of Bacillus megaterium spore coat proteins, characterising in-depth three novel proteins unique to this species. Graphical Abstract Figure. The first proteomic analysis of Bacillus megaterium spore coat proteins, characterising in-depth three novel proteins unique to this species.
ISSN:0378-1097
1574-6968
DOI:10.1111/1574-6968.12520