Amplified immunoassay ELISA-ELCA for measuring Clostridium botulinum type E neurotoxin in fish fillets

The measurement of Clostridium botulinum type E toxin in fish was accomplished using an amplified immunoassay (enzyme-linked immunosorbent assay-enzyme-linked coagulation assay [ELISA-ELCA]) based on the coagulation cascade. Fresh catfish fillets inoculated with a mixture of spores from five strains...

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Bibliographic Details
Published in:Journal of food protection 1994-11, Vol.57 (11), p.985-990
Main Authors: Roman, M.G. (Kraft General Foods, Inc., Glenview, Illinois), Humber, J.Y, Hall, P.A, Reddy, N.R, Solomon, H.M, Triscott, M.X, Beard, G.A, Bottoms, J.D, Cheng, T, Doellgast, G.J
Format: Article
Language:English
Subjects:
Biological and medical sciences
CLOSTRIDIUM BOTULINUM
COAGULACION
COAGULATION
ELISA
Food industries
Food toxicology
Freshwater
Fundamental and applied biological sciences. Psychology
Ictalurus punctatus
MEDICION
MESURE
PESCADO
POISSON (ALIMENT)
SISTEMA NERVIOSO
SYSTEME NERVEUX
TECHNIQUE IMMUNOLOGIQUE
TECNICAS INMUNOLOGICAS
TEST ELISA
TOXINAS
TOXINE
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Summary:The measurement of Clostridium botulinum type E toxin in fish was accomplished using an amplified immunoassay (enzyme-linked immunosorbent assay-enzyme-linked coagulation assay [ELISA-ELCA]) based on the coagulation cascade. Fresh catfish fillets inoculated with a mixture of spores from five strains of C. botulinum type E were packaged in high barrier film with air, vacuum and modified atmosphere and stored at 4, 8 or 16 degrees C for up to 75 days. Toxin production was monitored during storage by both mouse bioassay (trypsin and non-trypsin treated) and ELISA-ELCA on the non-trypsinized samples. All 26 inoculated products that were positive by the mouse bioassay were also positive by ELISA-ELCA. Of 35 uninoculated samples which were not toxic in mouse bioassay, none were positive by ELISA-ELCA; of 73 inoculated samples which were not toxic by mouse bioassay, 14 had toxin measurable by the ELISA-ELCA. The position of these immunoassay-positives in the sampling sequence indicated that the toxin was identified by the immunoassay before it was found in the mouse bioassay. These results suggest that the ELISA-ELCA technique is a usable alternative to the mouse bioassay for monitoring C. botulinum type E toxin production in fish challenge studies
ISSN:0362-028X
1944-9097
DOI:10.4315/0362-028X-57.11.985