Defense against lethal treatments and de novo protein synthesis induced by NaCl in Enterococcus faecalis ATCC 19433

Enterococcus faecalis was strongly resistant to high osmotic pressure in complex medium; however, when it was subjected to a moderate osmotic stress [6.5% (w/v) NaCl or 52% (w/v) sucrose] for 2 h, it showed cross-protection against ethanol (22%), detergents stresses [bile sales (0.3%) and SDS (0.017...

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Bibliographic Details
Published in:Archives of microbiology 1996-05, Vol.165 (5), p.317-324
Main Authors: Flahaut, S, Benachour, A, Giard, J C, Boutibonnes, P, Auffray, Y
Format: Article
Language:English
Subjects:
Adaptation, Physiological
Bacterial Proteins - biosynthesis
Bile Acids and Salts - pharmacology
Chloramphenicol - pharmacology
Electrophoresis, Gel, Two-Dimensional
Enterococcus faecalis
Enterococcus faecalis - drug effects
Enterococcus faecalis - growth & development
Enterococcus faecalis - metabolism
Ethanol - pharmacology
Heat-Shock Proteins - biosynthesis
Hydrogen Peroxide - pharmacology
Osmotic Pressure
Protein Synthesis Inhibitors - pharmacology
Sodium Chloride - pharmacology
Sodium Dodecyl Sulfate - pharmacology
Sucrose - pharmacology
Temperature
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Summary:Enterococcus faecalis was strongly resistant to high osmotic pressure in complex medium; however, when it was subjected to a moderate osmotic stress [6.5% (w/v) NaCl or 52% (w/v) sucrose] for 2 h, it showed cross-protection against ethanol (22%), detergents stresses [bile sales (0.3%) and SDS (0.017%)], hydrogen peroxide challenge (45 mM), and to a minor extent against lethal temperature (62 degrees C). In response to salt stress [6.5% (w/v) NaCl], E. faecalis induced a large number of stress proteins. In addition, NaCl strongly induced the synthesis of many proteins more than tenfold. Although the acquired thermotolerance was inhibited markedly by chloramphenicol, the other NaCl-induced cross-tolerances seemed not to be correlated with de novo protein synthesis. The relationship between the stress protein synthesis and the induction of different types of cross-protection is discussed.
ISSN:0302-8933
DOI:10.1007/s002030050333