Bacteria Community Study of Combined Periodontal‐Endodontic Lesions Using Denaturing Gradient Gel Electrophoresis and Sequencing Analysis

Background: The entire microbial population and predominant microflora of root canals (RCs) and adjacent periodontal pockets (PPs) from teeth with combined periodontal‐endodontic lesions were determined and compared. Methods: Pooled RC and PP samples were collected from the molars of 20 patients dia...

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Bibliographic Details
Published in:Journal of periodontology (1970) 2014-10, Vol.85 (10), p.1442-1449
Main Authors: Li, Hong, Guan, Rui, Sun, Jinghua, Hou, Benxiang
Format: Article
Language:English
Subjects:
Adult
Bacteria
Bacteroides - classification
denaturing gradient gel electrophoresis
Denaturing Gradient Gel Electrophoresis - methods
Dental Pulp Cavity - microbiology
Dental Pulp Diseases - microbiology
Dentistry
DNA, Bacterial - analysis
endodontics
Female
Fusobacterium - classification
gingival pocket
Humans
Male
Microbiota
Middle Aged
Molar - microbiology
Peptostreptococcus - classification
Periodontal Pocket - microbiology
periodontics
Polymerase Chain Reaction - methods
Porphyromonas - classification
Porphyromonas gingivalis - isolation & purification
sequence analysis
Sequence Analysis, DNA
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Summary:Background: The entire microbial population and predominant microflora of root canals (RCs) and adjacent periodontal pockets (PPs) from teeth with combined periodontal‐endodontic lesions were determined and compared. Methods: Pooled RC and PP samples were collected from the molars of 20 patients diagnosed with combined periodontal‐endodontic lesions. DNA was extracted for polymerase chain reaction–based denaturing gradient gel electrophoresis (PCR‐DGGE), cloning, and sequence analysis. A coefficient of similarity (Cs) was used to determine the similarity of the bacterial profiles from RCs and PPs. Results: Significantly fewer bands were produced by PCR‐DGGE from RCs (5.9 ± 1.7) than from PPs (8.0 ± 1.8) (P
ISSN:0022-3492
1943-3670
DOI:10.1902/jop.2014.130572