Rapid Detection of Immunity Against Bacteria in Asian Honeybee and Western Honeybee with Quantification of Royalisin in the Hemolymphe by Fast ELISA

Royalisin from royal jelly (RJ) is a valuable peptide both for the prevention of honeybee diseases and for RJ preservation. ELISA for fast determination of royalisin content in hemolymphe (RCH) of honeybees with polyclonal antibody against recombinant royalisin from Asian honeybee was established. A...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2014-09, Vol.62 (38), p.9305-9309
Main Authors: Shen, Li-Rong, Dilireba, Shatar, Zhou, Wen-Xiu, Wang, Yi-Ran, Li, Mei-Lu, Zhai, Liang
Format: Article
Language:English
Subjects:
Animals
bacteria
Bacteria - immunology
Bees - chemistry
Bees - classification
Bees - immunology
Bees - microbiology
enzyme-linked immunosorbent assay
Enzyme-Linked Immunosorbent Assay - methods
Hemolymph - chemistry
Hemolymph - immunology
honey bees
immune response
Immunity
innate immunity
pathogens
polyclonal antibodies
Proteins - analysis
Proteins - immunology
rapid methods
royal jelly
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Summary:Royalisin from royal jelly (RJ) is a valuable peptide both for the prevention of honeybee diseases and for RJ preservation. ELISA for fast determination of royalisin content in hemolymphe (RCH) of honeybees with polyclonal antibody against recombinant royalisin from Asian honeybee was established. Assay on RCHs of health samples from Asian honeybee and Western honeybee showed the former (7.06 μg/mL) was significantly higher than that of the latter (5.64 μg/mL, p < 0.01). Moreover, relative to the non infection, the RCHs of Asian honeybees at 24 and 48 h post infection of Eschericha coli were higher than those of Western honeybees by 32.90% and 29.66%, respectively. Evidence revealed that Asian honeybee possesses higher innate immunity and immune response against bacteria in relation to the Western honeybee. The method will be a potential tool for detection of resistant levels to pathogens in honeybees and for quantification of royalisin in RJ products.
ISSN:0021-8561
1520-5118
DOI:10.1021/jf501637g