In Situ Label-Free Visualization of Orally Dosed Strictinin within Mouse Kidney by MALDI-MS Imaging

Matrix-assisted laser desorption/ionization–mass spectrometry imaging (MALDI-MSI) is a powerful technique for visualizing the distribution of a wide range of biomolecules within tissue sections. However, methodology for visualizing a bioactive ellagitannin has not yet been established. This paper pr...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2014-09, Vol.62 (38), p.9279-9285
Main Authors: Kim, Yoon Hee, Fujimura, Yoshinori, Sasaki, Masako, Yang, Xue, Yukihira, Daichi, Miura, Daisuke, Unno, Yumi, Ogata, Koretsugu, Nakajima, Hiroki, Yamashita, Shuya, Nakahara, Kanami, Murata, Motoki, Lin, I-Chian, Wariishi, Hiroyuki, Yamada, Koji, Tachibana, Hirofumi
Format: Article
Language:English
Subjects:
Animals
biochemical compounds
Camellia sinensis - metabolism
ferulic acid
green tea
image analysis
Kidney - chemistry
Kidney - metabolism
kidneys
Male
Mice
Mice, Inbred BALB C
Molecular Structure
Phenols - administration & dosage
Phenols - chemistry
Phenols - metabolism
Plant Extracts - administration & dosage
Plant Extracts - chemistry
Plant Extracts - metabolism
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
spectroscopy
Tandem Mass Spectrometry - methods
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Summary:Matrix-assisted laser desorption/ionization–mass spectrometry imaging (MALDI-MSI) is a powerful technique for visualizing the distribution of a wide range of biomolecules within tissue sections. However, methodology for visualizing a bioactive ellagitannin has not yet been established. This paper presents a novel in situ label-free MALDI-MSI technique for visualizing the distribution of strictinin, a bioactive ellagitannin found in green tea, within mammalian kidney after oral dosing. Among nine representative matrix candidates, 1,5-diaminonaphthalene (1,5-DAN), harmane, and ferulic acid showed higher sensitivity to strictinin spotted onto a MALDI sample plate. Of these, 1,5-DAN enables visualization of a two-dimensional image of strictinin directly spotted on mouse kidney sections with the highest sensitivity. Furthermore, 1,5-DAN-based MALDI-MSI could detect the unique distribution of orally dosed strictinin within kidney sections. This in situ label-free imaging technique will contribute to the localization analysis of strictinin and its biological mechanisms.
ISSN:0021-8561
1520-5118
DOI:10.1021/jf503143g