In Situ Label-Free Visualization of Orally Dosed Strictinin within Mouse Kidney by MALDI-MS Imaging

• Published in: Journal of agricultural and food chemistry 2014-09, Vol.62 (38), p.9279-9285
• Main Authors: Kim, Yoon Hee, Fujimura, Yoshinori, Sasaki, Masako, Yang, Xue, Yukihira, Daichi, Miura, Daisuke, Unno, Yumi, Ogata, Koretsugu, Nakajima, Hiroki, Yamashita, Shuya, Nakahara, Kanami, Murata, Motoki, Lin, I-Chian, Wariishi, Hiroyuki, Yamada, Koji, Tachibana, Hirofumi
• Format: Article
• Language: English
• Subjects: Animals; biochemical compounds; Camellia sinensis - metabolism; ferulic acid; green tea; image analysis; Kidney - chemistry; Kidney - metabolism; kidneys; Male; Mice; Mice, Inbred BALB C; Molecular Structure; Phenols - administration & dosage; Phenols - chemistry; Phenols - metabolism; Plant Extracts - administration & dosage; Plant Extracts - chemistry; Plant Extracts - metabolism; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods; spectroscopy; Tandem Mass Spectrometry - methods
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf503143g

Matrix-assisted laser desorption/ionization–mass spectrometry imaging (MALDI-MSI) is a powerful technique for visualizing the distribution of a wide range of biomolecules within tissue sections. However, methodology for visualizing a bioactive ellagitannin has not yet been established. This paper presents a novel in situ label-free MALDI-MSI technique for visualizing the distribution of strictinin, a bioactive ellagitannin found in green tea, within mammalian kidney after oral dosing. Among nine representative matrix candidates, 1,5-diaminonaphthalene (1,5-DAN), harmane, and ferulic acid showed higher sensitivity to strictinin spotted onto a MALDI sample plate. Of these, 1,5-DAN enables visualization of a two-dimensional image of strictinin directly spotted on mouse kidney sections with the highest sensitivity. Furthermore, 1,5-DAN-based MALDI-MSI could detect the unique distribution of orally dosed strictinin within kidney sections. This in situ label-free imaging technique will contribute to the localization analysis of strictinin and its biological mechanisms.