Initial and total activity of rubisco in Scenedesmus ecornis [Chlorophyceae]: Is the french press a suitable tool for enzyme extraction?

An enzyme assay was developed to measure the initial and CO2-Mg2+ activated forms of Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) in the green microalga Scenedesmus ecornis. Comparisons were made of extraction by mortar and pestle versus the French press, in the presence or absence of N...

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Bibliographic Details
Published in:Plant and cell physiology 1993-03, Vol.34 (2), p.281-288
Main Authors: Mouget, J.L. (Universite Laval, Quebec (Canada)), Legendre, L, De La Nouee, J
Format: Article
Language:English
Subjects:
ACTIVIDAD ENZIMATICA
ACTIVITE ENZYMATIQUE
Biological and medical sciences
BIOTECHNOLOGIE
BIOTECHNOLOGY
BIOTECNOLOGIA
CHLOROPHYCEAE
Deactivation
ENZIMAS
ENZYME
enzyme activity
ENZYME PREPARATIONS
ENZYMES
ENZYMIC ACTIVITY
EXTRACTOS
EXTRACTS
EXTRAIT
French press
Fundamental and applied biological sciences. Psychology
measurement
Metabolism
NADPH
photosynthesis
Plant physiology and development
PREPARACION ENZIMATICA
PREPARATION ENZYMATIQUE
RIBULOSA-BIFOSFATO CARBOXILASA
RIBULOSE-BISPHOSPHATE CARBOXYLASE
Rubisco initial
Scenedesmus
Scenedesmus ecornis
Total activity
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Summary:An enzyme assay was developed to measure the initial and CO2-Mg2+ activated forms of Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) in the green microalga Scenedesmus ecornis. Comparisons were made of extraction by mortar and pestle versus the French press, in the presence or absence of NADPH. Losses due to harvesting and extraction procedures decreased when the algae were collected under the same irradiance they had experienced during growth, and by adding NADPH to the extraction solution. Results are discussed in relation to the possible interactions of effectors with the enzyme. The suitability of the French press to obtain catalytic competent extract is questioned.
ISSN:0032-0781
1471-9053
1471-9053
DOI:10.1093/oxfordjournals.pcp.a078418