The Development and Use of a DNA Polymerase Arrest Assay for the Evaluation of Parameters Affecting Intrastrand Tetraplex Formation

We show here that a K+-dependent block to DNA synthesis is a sensitive and specific indicator of intrastrand tetraplex formation that can be used, both to identify sequences with tetraplex-forming potential and to examine parameters that affect tetraplex formation. We show that tetraplex formation i...

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Bibliographic Details
Published in:The Journal of biological chemistry 1996-08, Vol.271 (34), p.20958-20964
Main Authors: Weitzmann, M. Neale, Woodford, Kerry J., Usdin, Karen
Format: Article
Language:English
Subjects:
Base Composition
Cell-Free System
DNA - biosynthesis
DNA - chemistry
DNA - metabolism
DNA - ultrastructure
DNA, Single-Stranded - chemistry
DNA-Directed DNA Polymerase - metabolism
Molecular Sequence Data
Nucleic Acid Conformation - drug effects
Oligodeoxyribonucleotides - chemistry
Potassium - chemistry
Sulfuric Acid Esters - chemistry
Templates, Genetic
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Summary:We show here that a K+-dependent block to DNA synthesis is a sensitive and specific indicator of intrastrand tetraplex formation that can be used, both to identify sequences with tetraplex-forming potential and to examine parameters that affect tetraplex formation. We show that tetraplex formation is determined by a complex combination of factors including the size and base composition of its constituent loops and stems. In the process of carrying out this study we have found that the number of sequences with the ability to form tetraplexes is larger than previously thought, and that such sequences are ubiquitous in eukaryote genomes.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.271.34.20958