Diversity of mercury resistance plasmids obtained by exogenous isolation from the bacteria of sugar beet in three successive years

• Published in: FEMS microbiology ecology 1996-08, Vol.20 (4), p.211-227
• Main Authors: Lilley, A.K, Bailey, M.J, Day, M.J, Fry, J.C
• Format: Article
• Language: English
• Subjects: Antibiotics; Bacteria; Beta vulgaris; Biological and medical sciences; Biotechnology; Carbon sources; characterization; Conjugation; Detoxification; diversity; Ecology; Environment and pollution; environmental factors; Fundamental and applied biological sciences. Psychology; gene transfer; Genetically engineered organisms behavior (microorganisms, plants, animals); Heavy metals; Horizontal gene transfer; Industrial applications and implications. Economical aspects; leaves; Mercury; Mercury (metal); Mercury resistance; metal tolerance; Microbiology; molecular diversity; Numerical analysis; persistence; Phyllosphere; Physiological effects; Plant growth; Plasmid classification; Plasmids; Pseudomonads; Pseudomonas; Pseudomonas putida; Reductase; Reductases; resistance; Rhizoplane; roots; self-transmissible plasmids; Sugar
• ISSN: 0168-6496; 1574-6941
• DOI: 10.1111/j.1574-6941.1996.tb00320.x

Self-transmissible plasmids conferring mercury resistance were exogenously isolated from the bacterial populations of sugar beet roots (rhizoplane) and leaves (phyllosphere) into a Pseudomonas putida recipient. Fifty rhizoplane plasmids and 29 phyllosphere plasmids (60-383 kb) were purified. Numerical analysis of plasmid DNA restriction enzyme digest patterns identified five distinct groups. Three of these plasmid groups were isolated from sugar beet crops grown at the same site over three consecutive years, demonstrating their established presence. Each group of plasmids comprised individual isolates with structural additions or deletions. The frequency of exogenous isolation correlated with factors likely to influence plant growth, bacterial activity and the physiological state of donors prior to sampling. All plasmids investigated conferred narrow spectrum mercury resistance with a reductase detoxification mechanism. None of the plasmids conferred resistance to a range of antibiotics, other heavy metals, or to UV, and following transfer to recipient bacteria the range of carbon source utilisation was not altered. This is the first report of the persistence of Pseudomonas spp. plasmid structural types isolated over several years from a terrestrial habitat.