Genomic organization of the entomopathogenic bacterium Bacillus thuringiensis subsp. berliner 1715

• Published in: Microbiology (Society for General Microbiology) 1996-07, Vol.142 (7), p.1625-1634
• Main Authors: Carlson, Cathrine Rein, Johansen, Trine, Lecadet, Marguerite-M, Kolsto, Anne-Brit
• Format: Article
• Language: English
• Subjects: Bacteriology; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Genetics; Microbiology
• ISSN: 1350-0872; 1465-2080
• DOI: 10.1099/13500872-142-7-1625

Institute of Pharmacy and Biotechnology Centre of Oslo, University of Oslo, PB 1125, 0316 Oslo, Norway Batteries Entomopathogenes, Institut Pasteur, 28 rue du Dr Roux, 75724 Paris Cedex 15, France 3 Author for correspondence: Anne-Brit Kolsto. Tel: [phone]. Fax: [phone]. e-mail: [e-mail] ABSTRACT A physical and genetic map of the Bacillus thuringiensis subsp. berliner 1715 (serotype 1) chromosome was constructed by pulsed field gel electrophoresis using three restriction enzymes, Asc l, Not l and Sfi l. A total of 24 restriction enzyme sites and 28 probes were located on the map. The chromosome size was 5.7 Mb. Consistent with previous mapping of Bacillus cereus chromosomes, the genes were nonrandomly distributed. Genes which are often plasmid-encoded were located on one half of the chromosome, whereas the other half contained rRNA genes and the origin region. Hybridization with macro-restriction fragments showed that the region containing rRNA genes and the origin was similar to that of the B. cereus type strain, ATCC 14579, and confirmed that the region was conserved between the B. cereus and B. thuringiensis chromosomes. The insecticidal toxin probe crylA or the transposon probe Tn4430 hybridized to six extrachromosomal elements of 60, 60, 100, 130, 270 and 600 kb, indicating that the genome size was at least 6.9 Mb. Keywords: Bacillus thuringiensis , physical mapping, transposon, insecticidal genes, pulsed field gel electrophoresis