Isolation and molecular analysis of the gene for cytochrome c sub(1) from Kluyveromyces lactis

By ethyl methanesulphonate mutagenesis of the yeast Kluyveromyces lactis we have isolated five nuclear mutants that were unable to grow on non-fermentable carbon sources. The mutations were found to belong to three complementation groups. After functional complementation of the mutation in one of th...

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Bibliographic Details
Published in:Current genetics 1996-01, Vol.30 (2), p.145-150
Main Authors: Gbelska, Y, Horvathova, K, Van der Aart, QJM, Zonneveld, BJM, Steensma, HY, Subik, J
Format: Article
Language:English
Subjects:
Kluyveromyces lactis
Saccharomyces cerevisiae
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Summary:By ethyl methanesulphonate mutagenesis of the yeast Kluyveromyces lactis we have isolated five nuclear mutants that were unable to grow on non-fermentable carbon sources. The mutations were found to belong to three complementation groups. After functional complementation of the mutation in one of these mutants we have cloned the structural gene for cytochrome c sub(1), named KlCYT1. This gene has been assigned to chromosome VI and its nucleotide sequence exhibited 74.3% identity to the homologous gene of S. cerevisiae.
ISSN:0172-8083