Crucial surviving aspects for vitrified in vitro-produced bovine embryos

The objective of the present study was to correlate some parameters (cleavage, blastocyst production, quality degree score, total cell number, fresh apoptosis and lipid content) with embryo survival after cryopreservation. A total of 1727 in vitro-produced bovine blastocysts were used to establish t...

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Bibliographic Details
Published in:Zygote (Cambridge) 2014-05, Vol.22 (2), p.124-131
Main Authors: Sudano, Mateus José, Paschoal, Daniela Martins, da Silva Rascado, Tatiana, Crocomo, Letícia Ferrari, Magalhães, Luis Carlos Oña, Junior, Alício Martins, Machado, Rui, da Cruz Landim-Alvarenga, Fernanda
Format: Article
Language:English
Subjects:
Animals
Apoptosis
Blastocyst - cytology
Blastocyst - physiology
Cattle - embryology
Cattle - metabolism
Cell Survival
Cryopreservation - methods
Cryopreservation - veterinary
Embryo Culture Techniques
Embryo, Mammalian - cytology
Embryo, Mammalian - physiology
Female
Fertilization in Vitro
In Vitro Techniques
Lipids - analysis
Vitrification
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Summary:The objective of the present study was to correlate some parameters (cleavage, blastocyst production, quality degree score, total cell number, fresh apoptosis and lipid content) with embryo survival after cryopreservation. A total of 1727 in vitro-produced bovine blastocysts were used to establish the parameters (mean ± standard error of the mean (SEM)) for cleavage (85.6 ± 0.8), blastocyst production (39.9 ± 1.4), quality degree score (1.6 ± 0.1), total cell number (140.1 ± 2.9), fresh apoptosis (20.8 ± 1.1) and lipid content (21.3 ± 0.8 droplets). On the same way 1316 blastocysts were vitrified for the determination of post-cryopreservation embryo survival (49.4 ± 1.9). Fresh apoptosis rate and total lipid droplets value were correlated (P < 0.05) with embryo survival after cryopreservation (r = 0.91 and r = 0.59; respectively). However, cleavage, blastocyst production, quality degree score and total cell number were not correlated (P > 0.05) with embryo cryotolerance (r = 0.23, r = 0.38, r = 0.22 and r = 0.28; respectively). Therefore, the increased lipid content was moderately correlated with apoptosis in vitrified blastocysts. On the other hand, increased apoptosis in fresh blastocysts was strongly correlated with apoptosis in vitrified blastocysts, which indicated that the apoptosis rate in fresh embryos was a better parameter than the lipid content to predict post-vitrification embryo survival.
ISSN:0967-1994
1469-8730
DOI:10.1017/S0967199412000196