Possible regulation of Oct60 transcription by a positive feedback loop in Xenopus oocytes

The POU family subclass V (POU-V) proteins have important roles in maintaining cells in an undifferentiated state. In Xenopus, expression of the POU-V protein Oct60 was detected in oocytes and was found to decrease in blastula- to gastrula-stage embryos. In addition, Oct60 overexpression inhibits so...

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Bibliographic Details
Published in:Zygote (Cambridge) 2014-05, Vol.22 (2), p.266-274
Main Authors: Morichika, Keisuke, Sugimoto, Makoto, Yasuda, Katsuki, Kinoshita, Tsutomu
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Blastula - cytology
Blastula - physiology
Chromatin Immunoprecipitation
Embryo, Nonmammalian - cytology
Embryo, Nonmammalian - physiology
Feedback, Physiological
Female
Gastrula - cytology
Gastrula - physiology
Gene Expression Regulation, Developmental
Luciferases
Molecular Sequence Data
Oocytes - cytology
Oocytes - physiology
POU Domain Factors
Promoter Regions, Genetic - genetics
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - genetics
Sequence Homology, Nucleic Acid
Signal Transduction
Transcription, Genetic - genetics
Xenopus
Xenopus laevis - embryology
Xenopus laevis - genetics
Xenopus laevis - metabolism
Xenopus Proteins - genetics
Xenopus Proteins - metabolism
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Summary:The POU family subclass V (POU-V) proteins have important roles in maintaining cells in an undifferentiated state. In Xenopus, expression of the POU-V protein Oct60 was detected in oocytes and was found to decrease in blastula- to gastrula-stage embryos. In addition, Oct60 overexpression inhibits some signals in early embryogenesis, including Activin/Nodal, BMP, and Wnt signalling. In this report, we analysed mechanisms of Oct60 promoter activation and discovered that Oct60 transcription was activated ectopically in somatic nuclei by oocyte extract treatment. Promoter assays demonstrated that Oct60 transcription was activated in oocytes specifically and that this activation was dependent on an Octamer-Sox binding motif. ChIP assays showed that the Oct60 protein binds the motif. These results suggest that Oct60 transcription is regulated by a positive-feedback loop in Xenopus oocytes.
ISSN:0967-1994
1469-8730
DOI:10.1017/S0967199412000536