Regulatory T cell subtypes and TGF-β1 gene expression in chronic allograft dysfunction

Regulatory T cells have been suggested to have a protective role against acute rejection in allograft recipients. However, there is little information available about their contribution to chronic rejection process. The role of transforming growth factor-beta 1 (TGF-β1) as a profibrogenic and/or imm...

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Bibliographic Details
Published in:Iranian journal of immunology 2014-09, Vol.11 (3), p.139-152
Main Authors: Assadiasl, Sara, Ahmadpoor, Pedram, Nafar, Mohsen, Lessan Pezeshki, Mahboob, Pourrezagholi, Fateme, Parvin, Mahmoud, Shahlaee, Abtin, Sepanjnia, Adel, Nicknam, Mohammad Hossein, Amirzargar, Aliakbar
Format: Article
Language:English
Subjects:
Adult
Allografts - metabolism
Allografts - pathology
Antigens, Surface - metabolism
Case-Control Studies
Creatinine - blood
Creatinine - urine
Female
Gene Expression
Humans
Immunophenotyping
Kidney Transplantation - adverse effects
Lymphocyte Count
Male
Middle Aged
Renal Insufficiency, Chronic - genetics
Renal Insufficiency, Chronic - pathology
T-Lymphocyte Subsets - immunology
T-Lymphocyte Subsets - metabolism
T-Lymphocytes, Regulatory - immunology
T-Lymphocytes, Regulatory - metabolism
Transforming Growth Factor beta1 - genetics
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Summary:Regulatory T cells have been suggested to have a protective role against acute rejection in allograft recipients. However, there is little information available about their contribution to chronic rejection process. The role of transforming growth factor-beta 1 (TGF-β1) as a profibrogenic and/or immunoregulatory cytokine in renal allografts is also controversial. To evaluate the frequency of CD4+CD25+CD127- and CD3+CD8+CD28- regulatory T cells in chronic allograft dysfunction (CAD) and to investigate the expression of TGF-β1 in renal allografts. Thirty biopsy-proven CAD patients were pair-matched with 30 stable graft function patients and a third group of healthy volunteers. Flowcytometry was performed on PBMCs to determine the frequency of CD3+CD8+CD28- and CD4+CD25+CD127- regulatory T cells in lymphocyt population. TGF-β1 gene expression was assessed by Real Time PCR. The percentages of CD3+CD8+CD28- Tregs among renal allograft recipients was higher than healthy controls (p
ISSN:1735-367X