Optimal production of 4-deoxy-l-erythro-5-hexoseulose uronic acid from alginate for brown macro algae saccharification by combining endo- and exo-type alginate lyases

Algae are considered as third-generation biomass, and alginate is the main component of brown macroalgae. Alginate can be enzymatically depolymerized by alginate lyases into uronate monomers, such as mannuronic acid and guluronic acid, which are further nonenzymatically converted to 4-deoxy- l -eryt...

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Bibliographic Details
Published in:Bioprocess and biosystems engineering 2014-10, Vol.37 (10), p.2105-2111
Main Authors: Wang, Da Mao, Kim, Hee Taek, Yun, Eun Ju, Kim, Do Hyoung, Park, Yong-Cheol, Woo, Hee Chul, Kim, Kyoung Heon
Format: Article
Language:English
Subjects:
Acids
Algae
Alginates - metabolism
Biofuels
Biotechnology
Chemistry
Chemistry and Materials Science
Chromatography, Thin Layer
Deoxy Sugars - biosynthesis
Environmental Engineering/Biotechnology
Food Science
Gas chromatography
Gas Chromatography-Mass Spectrometry
Glucuronic Acid - metabolism
Hexuronic Acids - metabolism
Industrial and Production Engineering
Industrial Chemistry/Chemical Engineering
Mass spectrometry
Oligosaccharides - biosynthesis
Optimization techniques
Original Paper
Phaeophyceae - metabolism
Polysaccharide-Lyases - metabolism
Thin layer chromatography
Uronic Acids - metabolism
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Summary:Algae are considered as third-generation biomass, and alginate is the main component of brown macroalgae. Alginate can be enzymatically depolymerized by alginate lyases into uronate monomers, such as mannuronic acid and guluronic acid, which are further nonenzymatically converted to 4-deoxy- l -erythro-5-hexoseulose uronic acid (DEH). We have optimized an enzymatic saccharification process using two recombinant alginate lyases, endo-type Alg7D and exo-type Alg17C, for the efficient production of DEH from alginate. When comparing the sequential and simultaneous additions of Alg7D and Alg17C, it was found that the final yield of DEH was significantly higher when the enzymes were added sequentially. The progress of saccharification reactions and production of DEH were verified by thin layer chromatography and gas chromatography–mass spectrometry, respectively. Our results showed that the two recombinant enzymes could be exploited for the efficient production of DEH that is the key substrate for producing biofuels from brown macro algal biomass.
ISSN:1615-7591
1615-7605
DOI:10.1007/s00449-014-1188-3