Preparation of pre-cut corneas from fresh donated whole globes for Descemet’s stripping automated keratoplasty: 3-year results at the Central Eye Bank of Iran

To describe the technique and the results of the preparation of pre-cut corneas for Descemet’s stripping automated endothelial keratoplasty (DSAEK) during a 3-year period at the Central Eye Bank of Iran (CEBI). The method of preparation of pre-cut corneas from donated whole globes at the CEBI is des...

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Bibliographic Details
Published in:Cell and tissue banking 2014-09, Vol.15 (3), p.369-372
Main Authors: Kanavi, Mozhgan Rezaei, Javadi, Mohammad Ali, Javadi, Fatemeh, Chamani, Tahereh
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Biomedicine
Blood & organ donations
Cell Biology
Cornea
Cornea - surgery
Corneal Transplantation - methods
Descemet Membrane - surgery
Descemet Stripping Endothelial Keratoplasty - methods
Endothelium, Corneal - surgery
Eye Banks
Humans
Iran
Life Sciences
Original Paper
Tissue Donors
Tissue engineering
Transplant Surgery
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Summary:To describe the technique and the results of the preparation of pre-cut corneas for Descemet’s stripping automated endothelial keratoplasty (DSAEK) during a 3-year period at the Central Eye Bank of Iran (CEBI). The method of preparation of pre-cut corneas from donated whole globes at the CEBI is described and the frequency and percentage of pre-cut corneas prepared for DSAEK, between April 2009 and March 2012, are specified. Moreover, post-operative reports are reviewed for any complaints about using pre-cut tissues for DSAEK. Out of the 1,518 donated whole globes appropriate for DSAEK, 1,478 (97.4 %) pre-cut corneas were successfully prepared. The method of preparation failed in 40 (2.6 %) cases. Based on the eye bank post-operative reports, thickness of pre-cut tissues for DSAEK was deemed unacceptable in only 6 (0.4 %) cases prior to surgery; five of these were too thick and one was too thin. Preparation of pre-cut corneas, for DSAEK from donated whole globes, in the CEBI is a safe and easy method, with very good preservation of endothelial cells after the preparation of the pre-cut corneas and reduced risks from corneal manipulation.
ISSN:1389-9333
1573-6814
DOI:10.1007/s10561-013-9392-y