Aerobic Transformation of BDE-47 by a Pseudomonas putida sp. Strain TZ-1 Isolated from PBDEs-Contaminated Sediment

• Published in: Bulletin of environmental contamination and toxicology 2014-10, Vol.93 (4), p.483-488
• Main Authors: Xin, Jia, Liu, Xiang, Liu, Wei, Zheng, Xi-Lai
• Format: Article
• Language: English
• Subjects: Aquatic Pollution; Bacteria; Base Sequence; Biochemistry; Biodegradation; Biodegradation of pollutants; Biodegradation, Environmental; Biological and medical sciences; Biotechnology; Biotransformation; biphenyl; carbon; cell growth; Chemical contaminants; China; Cluster Analysis; Contaminated sediments; DNA Primers - genetics; Earth and Environmental Science; Ecotoxicology; Electronic waste; Electronic Waste - analysis; energy; Energy sources; Environment; Environment and pollution; Environmental Chemistry; Environmental Health; Fundamental and applied biological sciences. Psychology; Geologic Sediments - chemistry; Geologic Sediments - microbiology; Halogenated Diphenyl Ethers - analysis; Halogenated Diphenyl Ethers - metabolism; Hydrogen-Ion Concentration; Industrial applications and implications. Economical aspects; Kinetics; Molecular Sequence Data; Phylogeny; Pollution; Pseudomonas putida; Pseudomonas putida - genetics; Pseudomonas putida - metabolism; RNA, Ribosomal, 16S - genetics; Sediment pollution; sediments; sequence analysis; Sequence Analysis, DNA; Soil Pollutants - analysis; Soil Pollutants - metabolism; Soil Science & Conservation; Temperature; Toxicity; Waste Water Technology; Water Management; Water Pollution Control
• ISSN: 0007-4861; 1432-0800
• DOI: 10.1007/s00128-014-1306-6

A bacterial isolate, TZ-1, was isolated from contaminated sediment near electronic waste dismantling workshops, Taizhou, China that degraded 2,2′,4,4′-tetrabrominated diphenyl ether (BDE-47). The isolate was identified as Pseudomonas putida sp. with respect to its morphology, biochemical characteristics and 16SrDNA sequence analysis. TZ-1 can use BDE-47 as the sole carbon and energy source for growth in mineral salt medium. The isolate degraded BDE-47 up to 49.96 % of the initially applied concentration of 50 μg L⁻¹ after 7 days of incubation at 150 rpm, 30°C. Static conditions with pH 6.5 and temperature 30°C were considered to be optimum for BDE-47 biodegradation. Addition of co-substrates promoted cell growth, but decreased the degradation rate for BDE-47.