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Effects of Acute Exposure to the Non-steroidal Anti-inflammatory Drug Ibuprofen on the Developing North American Bullfrog (Rana catesbeiana) Tadpole

A variety of pharmaceutical chemicals can represent constituents of municipal effluent outflows that are dispersed into aquatic receiving environments worldwide. Increasingly, there is concern as to the potential of such bioactive substances to interact with wildlife species at sensitive life stages...

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Published in:Environmental science & technology 2014-09, Vol.48 (17), p.10439-10447
Main Authors: Veldhoen, Nik, Skirrow, Rachel C, Brown, Lorraine L. Y, van Aggelen, Graham, Helbing, Caren C
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cited_by cdi_FETCH-LOGICAL-a442t-f12a4fd336a55edad5d2c28390cd9b13f45953315e3845d39382e66378e75243
cites cdi_FETCH-LOGICAL-a442t-f12a4fd336a55edad5d2c28390cd9b13f45953315e3845d39382e66378e75243
container_end_page 10447
container_issue 17
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creator Veldhoen, Nik
Skirrow, Rachel C
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van Aggelen, Graham
Helbing, Caren C
description A variety of pharmaceutical chemicals can represent constituents of municipal effluent outflows that are dispersed into aquatic receiving environments worldwide. Increasingly, there is concern as to the potential of such bioactive substances to interact with wildlife species at sensitive life stages and affect their biology. Using a combination of DNA microarray, quantitative real-time polymerase chain reaction, and quantitative nuclease protection assays, we assessed the ability of sub-lethal and environmentally relevant concentrations of ibuprofen (IBF), a non-steroidal anti-inflammatory agent and prevalent environmental contaminant, to function as a disruptor of endocrine-mediated post-embryonic development of the frog. While the LC50 of IBF for pre-metamorphic Rana catesbeiana tadpoles is 41.5 mg/L (95% confidence interval: 32.3–53.5 mg/L), exposure to concentrations in the ppb range elicited molecular responses both in vivo and in organ culture. A nominal concentration of 15 μg/L IBF (actual = 13.7 μg/L) altered the abundance of 26 mRNA transcripts within the liver of exposed pre-metamorphic R. catesbeiana tadpoles within 6 d. IBF-treated animals demonstrated subsequent disruption of thyroid hormone-mediated reprogramming in the liver transcriptome affecting constituents of several metabolic, developmental, and signaling pathways. Cultured tadpole tail fin treated with IBF for 48 h also demonstrated altered mRNA levels at drug concentrations as low as 1.5 μg/L. These observations raise the possibility that IBF may alter the post-embryonic development of anuran species in freshwater environs, where IBF is a persistent or seasonal pollutant.
doi_str_mv 10.1021/es502539g
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ispartof Environmental science & technology, 2014-09, Vol.48 (17), p.10439-10447
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source American Chemical Society:Jisc Collections:American Chemical Society Read & Publish Agreement 2022-2024 (Reading list)
subjects Animal Fins - drug effects
Animal Fins - metabolism
Animals
Anti-Inflammatory Agents, Non-Steroidal - chemistry
Anti-Inflammatory Agents, Non-Steroidal - pharmacology
Effects
Embryology
Frogs
Gene Expression Regulation, Developmental - drug effects
Ibuprofen - chemistry
Ibuprofen - pharmacology
Larva - drug effects
Larva - genetics
Liver - drug effects
Liver - metabolism
Nonsteroidal anti-inflammatory drugs
North America
Polymerase chain reaction
Rana catesbeiana
Rana catesbeiana - genetics
Rana catesbeiana - growth & development
Real-Time Polymerase Chain Reaction
RNA, Messenger - metabolism
Transcriptome - drug effects
Transcriptome - genetics
Triiodothyronine - pharmacology
title Effects of Acute Exposure to the Non-steroidal Anti-inflammatory Drug Ibuprofen on the Developing North American Bullfrog (Rana catesbeiana) Tadpole
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