Time in hemodialysis modulates the levels of genetic damage in hemodialysis patients

It is assumed that hemodialysis treatment can diminish the levels of genetic damage in circulating lymphocytes by cleaning the blood of uremic toxins that cause oxidative stress. However, the hemodialysis process by itself may also induce genomic damage by producing reactive oxygen species (ROS). We...

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Bibliographic Details
Published in:Environmental and molecular mutagenesis 2014-05, Vol.55 (4), p.363-368
Main Authors: Rodríguez-Ribera, Lara, Stoyanova, Elitsa, Corredor, Zuray, Coll, Elisabet, Silva, Irene, Diaz, Juan Manuel, Ballarin, José, Marcos, Ricard, Pastor, Susana
Format: Article
Language:English
Subjects:
Aged
Cells, Cultured
CRF patients
DNA Damage - genetics
DNA Damage - radiation effects
Female
Follow-Up Studies
Genomic Instability
Humans
Kidney Failure, Chronic - blood
Kidney Failure, Chronic - genetics
Kidney Failure, Chronic - therapy
Lymphocytes - metabolism
Lymphocytes - pathology
Lymphocytes - radiation effects
Male
micronucleus assay
Micronucleus Tests
Oxidative Stress - radiation effects
radiosensitivity
Reactive Oxygen Species - metabolism
Renal Dialysis - adverse effects
uremic state
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Summary:It is assumed that hemodialysis treatment can diminish the levels of genetic damage in circulating lymphocytes by cleaning the blood of uremic toxins that cause oxidative stress. However, the hemodialysis process by itself may also induce genomic damage by producing reactive oxygen species (ROS). We conducted a follow‐up study in a group of 70 hemodialysis patients followed for a mean time of 15 months. We investigated the effect of exposure time in hemodialysis on the levels of genetic damage in peripheral blood lymphocytes using the micronucleus assay. In addition, genetic damage after in vitro irradiation with 0.5 Gy was also analyzed to evaluate changes in radiosensitivity. Our results showed that, at the end of the study, there was a decrease in both the basal levels of genetic damage (9.9 ± 1.0 vs. 7.6 ± 0.7) and radiosensitivity values (38.5 ± 3.0 vs. 27.6 ± 2.4). We conclude that hemodialysis procedures may act as an ameliorating factor reducing the genetic damage present in chronic kidney disease patients. Environ. Mol. Mutagen. 55:363–368, 2014. © 2014 Wiley Periodicals, Inc.
ISSN:0893-6692
1098-2280
DOI:10.1002/em.21849