complete genome of a putative endornavirus identified in yerba mate (Ilex paraguariensis St. Hil.)

We present the first report of a virus infecting the subtropical tree crop yerba mate (Ilex paraguariensis St. Hil.). Total RNA purification, followed by next-generation sequencing, transcripts assembly and annotation, resulted in the identification of a new endornavirus species infecting yerba mate...

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Bibliographic Details
Published in:Virus genes 2014-10, Vol.49 (2), p.348-350
Main Authors: Debat, Humberto J, Grabiele, Mauro, Aguilera, Patricia M, Bubillo, Rosana, Zapata, Pedro D, Marti, Dardo A, Ducasse, Daniel A
Format: Article
Language:English
Subjects:
3' Untranslated Regions
5' Untranslated Regions
Biomedical and Life Sciences
Biomedicine
Cluster Analysis
double-stranded RNA
Endornavirus
genome
Genome, Viral
high-throughput nucleotide sequencing
Ilex
Ilex paraguariensis
Ilex paraguariensis - virology
Medical Microbiology
Molecular Sequence Data
Open Reading Frames
Phylogeny
Plant Sciences
Plant Viruses - genetics
Plant Viruses - isolation & purification
polypeptides
Polyproteins - genetics
protein synthesis
RNA Viruses - genetics
RNA Viruses - isolation & purification
RNA, Double-Stranded - genetics
RNA, Viral - genetics
RNA-directed RNA polymerase
Sequence Analysis, DNA
Sequence Homology
trees
Virology
viruses
yerba mate
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Summary:We present the first report of a virus infecting the subtropical tree crop yerba mate (Ilex paraguariensis St. Hil.). Total RNA purification, followed by next-generation sequencing, transcripts assembly and annotation, resulted in the identification of a new endornavirus species infecting yerba mate. The complete sequence of the linear dsRNA viral genome is 13,954-nt long, contains a single 13,743 nt ORF, and presents a 149 nt 5′UTR and a 61 nt 3′UTR. The predicted ORF encodes a 4,581 aa polypeptide with a UDP-glucose glycosyl-transferase, a capsular polysaccharide synthesis protein, and a RNA-dependent RNA polymerase domain. The name yerba mate endornavirus is proposed for the identified virus. Due to the intriguing peculiarities of this virus family, and the complete lack of the yerba mate virus literature, we consider that the information reported here will be helpful in leading to a new and needed attention to this important topic and crop.
ISSN:0920-8569
1572-994X
DOI:10.1007/s11262-014-1096-2