Distribution of potato spindle tuber viroid in reproductive organs of petunia during its developmental stages

• Published in: Phytopathology 2014-09, Vol.104 (9), p.964-969
• Main Authors: Matsushita, Yosuke, Tsuda, Shinya
• Format: Article
• Language: English
• Subjects: Flowers - cytology; Flowers - growth & development; Flowers - physiology; Flowers - virology; In Situ Hybridization; Lycopersicon esculentum - virology; Meristem - cytology; Meristem - growth & development; Meristem - physiology; Meristem - virology; Petunia; Petunia - cytology; Petunia - growth & development; Petunia - physiology; Petunia - virology; Plant Diseases - virology; Plant Shoots - cytology; Plant Shoots - growth & development; Plant Shoots - physiology; Plant Shoots - virology; Plant Tubers - virology; Pollen - cytology; Pollen - growth & development; Pollen - physiology; Pollen - virology; Potato spindle tuber viroid; Reproduction; Seeds - cytology; Seeds - growth & development; Seeds - physiology; Seeds - virology; Viroids - physiology
• ISSN: 0031-949X; 1943-7684
• DOI: 10.1094/phyto-10-13-0294-r

Embryo infection is important for efficient seed transmission of viroids. To identify the major pattern of seed transmission of viroids, we used in situ hybridization to histochemically analyze the distribution of Potato spindle tuber viroid (PSTVd) in each developmental stage of petunia (flowering to mature seed stages). In floral organs, PSTVd was present in the reproductive tissues of infected female × infected male and infected female × healthy male but not of healthy female × infected male before embryogenesis. After pollination, PSTVd was detected in the developed embryo and endosperm in all three crosses. These findings indicate that PSTVd is indirectly delivered to the embryo through ovule or pollen during the development of reproductive tissues before embryogenesis but not directly through maternal tissues as cell-to-cell movement during embryogenesis.