Plant genome engineering in full bloom

•The CRISPR/Cas9 system is a versatile tool for genome engineering.•A customizable gRNA and a nuclease are the core components of the CRISPR/Cas9 system.•The CRISPR/Cas9 system has been validated in Arabidopsis, tobacco, rice, wheat, and sorghum.•Researchers should be aware of potential off-target m...

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Bibliographic Details
Published in:Trends in plant science 2014-05, Vol.19 (5), p.284-287
Main Authors: Lozano-Juste, Jorge, Cutler, Sean R.
Format: Article
Language:English
Subjects:
Biotechnology
Cas9
Clustered Regularly Interspaced Short Palindromic Repeats - genetics
CRISPR
CRISPR-Cas Systems - genetics
Endonucleases - genetics
Gene Expression Regulation, Plant
Gene Targeting - methods
Genetic Engineering - methods
genome engineering
Genome, Plant - genetics
Mutation
plant
Plants - genetics
Systems Biology
TALENs
ZFNs
Zinc Fingers
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Summary:•The CRISPR/Cas9 system is a versatile tool for genome engineering.•A customizable gRNA and a nuclease are the core components of the CRISPR/Cas9 system.•The CRISPR/Cas9 system has been validated in Arabidopsis, tobacco, rice, wheat, and sorghum.•Researchers should be aware of potential off-target mutations when using the CRISPR/Cas9 system. The recent development of tools for precise editing of user-specified sequences is rapidly changing the landscape for plant genetics and biotechnology. It is now possible to target mutations and regulatory proteins to specific sites in a genome using zinc-finger nucleases (ZFNs), transcription activator-like endonucleases (TALENs), or the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) system. Here we provide an update of recent developments in CRISPR/Cas9 technology and highlight online resources that will help biologists adopt new genome-editing tools.
ISSN:1360-1385
1878-4372
DOI:10.1016/j.tplants.2014.02.014