Using Amino-Labeled Nucleotide Probes for Simultaneous Single Molecule RNA-DNA FISH: e107425

Using amino-labeled oligonucleotide probes, we established a simple, robust and low-noise method for simultaneous detection of RNA and DNA by fluorescence in situ hybridization, a highly useful tool to study the large pool of long non-coding RNAs being identified in the current research. With probes...

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Bibliographic Details
Published in:PloS one 2014-09, Vol.9 (9)
Main Authors: Basu, Reelina, Lai, Lan-Tian, Meng, Zhenyu, Wu, Jun, Shao, Fangwei, Zhang, Li-Feng
Format: Article
Language:English
Online Access:Get full text
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Summary:Using amino-labeled oligonucleotide probes, we established a simple, robust and low-noise method for simultaneous detection of RNA and DNA by fluorescence in situ hybridization, a highly useful tool to study the large pool of long non-coding RNAs being identified in the current research. With probes either chemically or biologically synthesized, we demonstrate that the method can be applied to study a wide range of RNA and DNA targets at the single-cell and single-molecule level in cellular contexts.
ISSN:1932-6203
DOI:10.1371/journal.pone.0107425