Ultra-high throughput detection of single cell ?-galactosidase activity in droplets using micro-optical lens array

We demonstrate the use of a hybrid microfluidic-micro-optical system for the screening of enzymatic activity at the single cell level. Escherichia coli beta -galactosidase activity is revealed by a fluorogenic assay in 100 pl droplets. Individual droplets containing cells are screened by measuring t...

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Bibliographic Details
Published in:Applied physics letters 2013-01, Vol.103 (20)
Main Authors: Lim, Jiseok, Vrignon, Jeremy, Gruner, Philipp, Karamitros, Christos S, Konrad, Manfred, Baret, Jean-Christophe
Format: Article
Language:English
Subjects:
Arrays
Channels
Droplets
Escherichia coli
Flow cytometry
High speed
Mathematical analysis
Microlenses
Online Access:Get full text
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Summary:We demonstrate the use of a hybrid microfluidic-micro-optical system for the screening of enzymatic activity at the single cell level. Escherichia coli beta -galactosidase activity is revealed by a fluorogenic assay in 100 pl droplets. Individual droplets containing cells are screened by measuring their fluorescence signal using a high-speed camera. The measurement is parallelized over 100 channels equipped with microlenses and analyzed by image processing. A reinjection rate of 1 ml of emulsion per minute was reached corresponding to more than 105 droplets per second, an analytical throughput larger than those obtained using flow cytometry.
ISSN:0003-6951
DOI:10.1063/1.4830046