Activation of c-Jun-NH sub(2)-kinase by UV irradiation is dependent on p21 super(ras)

We have demonstrated previously that Jun-NH sub(2)-kinase (JNK) activation in vitro is potentiated by association with the p21 super(ras) protein. To determine if in vivo activation of JNK also depends on p21 super(ras), we have used M1311 cells that carry the cDNA for the neutralizing antibody to p...

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Bibliographic Details
Published in:The Journal of biological chemistry 1996-01, Vol.271 (38), p.23304-23309
Main Authors: Adler, V, Pincus, M R, Polotskaya, A, Montano, X, Freidman, F K, Ronai, Z
Format: Article
Language:English
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Summary:We have demonstrated previously that Jun-NH sub(2)-kinase (JNK) activation in vitro is potentiated by association with the p21 super(ras) protein. To determine if in vivo activation of JNK also depends on p21 super(ras), we have used M1311 cells that carry the cDNA for the neutralizing antibody to p21 super(ras), Y13-259, under a dexamethasone-inducible promoter. The ability of UV to activate JNK gradually decreased over a 4-day period of cell growth in dexamethasone. This decrease coincides with weaker transcriptional activation measured via gel shift and chloramphenicol acetyltransferase assays. Peptides corresponding to amino acids 96-110 on p21 super(ras), which were shown to block Ras-JNK association, inhibited UV-mediated JNK activation in mouse fibroblast 3T3-4A cells as well as in M1311 cells, further supporting the role of p21 super(ras) in UV-mediated JNK activation. Overall, the present studies provide in vivo confirmation of the role p21 super(ras) plays in JNK activation by UV irradiation.
ISSN:0021-9258