Near-field fluorescent imaging of single proteins

We report the results of our initial efforts to image photosynthetic proteins using the near-field technique. Allophycocyanin trimers, which contains six chromophores each, were dispersed onto a glass cover slip, and the fluorescence was imaged using a recently constructed near-field microscope. The...

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Bibliographic Details
Published in:Ultramicroscopy 1995-02, Vol.57 (2), p.113-117
Main Authors: Dunn, Robert C., Allen, Errol V., Joyce, Stephen A., Anderson, Gordon A., Sunney Xie, X.
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Non metallic chromoproteins, photoproteins
Proteins
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Summary:We report the results of our initial efforts to image photosynthetic proteins using the near-field technique. Allophycocyanin trimers, which contains six chromophores each, were dispersed onto a glass cover slip, and the fluorescence was imaged using a recently constructed near-field microscope. The results show that the emission from single proteins can be detected and localized on the nanometer scale.
ISSN:0304-3991
1879-2723
DOI:10.1016/0304-3991(94)00121-3