Use of a new rapid bioluminescence method for screening organophosphate and N-methylcarbamate insecticides in processed baby foods

An enzyme with high specific affinity for organophosphate and N-methylcarbamate insecticides has been incorporated into a new test for detection of these insecticides at the level of parts per billion (ppb) (commercially available as the Charm Pesticide Test). To measure the extent of insecticide in...

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Bibliographic Details
Published in:Journal of food protection 1996-03, Vol.59 (3), p.306-311
Main Authors: Saul, S.J. (Charm Sciences, Malden, MA.), Zomer, E, Puopolo, D, Charm, S.E
Format: Article
Language:English
Subjects:
ALIMENT POUR NOURRISSON
ALIMENTOS PARA NINOS
Biological and medical sciences
CARBAMATE
Carbamates - analysis
CARBAMATOS
CHROMATOGRAPHIE EN PHASE GAZEUSE
Chromatography, High Pressure Liquid
COMPOSE ORGANOPHOSPHORE
COMPUESTO ORGANICO DEL FOSFORO
CROMATOGRAFIA DE GASES
ENSAYO
ESPECTROMETRIA DE MASAS
Food Contamination
Food industries
Food Inspection - methods
Fundamental and applied biological sciences. Psychology
Gas Chromatography-Mass Spectrometry
HPLC
INDUSTRIA ALIMENTARIA
INDUSTRIE ALIMENTAIRE
Infant Food - analysis
Infant Food - standards
INNOCUITE DES PRODUITS ALIMENTAIRES
INOCUIDAD ALIMENTARIA
INSECTICIDAS
INSECTICIDE
Insecticides - analysis
Luminescent Measurements
Organophosphorus Compounds
Pesticide Residues - analysis
PROPIEDADES OPTICAS
PROPRIETE OPTIQUE
Research and development. New food products, dietetic foods and beverages
RESIDU
RESIDUOS
SPECTROMETRIE DE MASSE
TECHNIQUE ANALYTIQUE
TECNICAS ANALITICAS
TESTAGE
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Description
Summary:An enzyme with high specific affinity for organophosphate and N-methylcarbamate insecticides has been incorporated into a new test for detection of these insecticides at the level of parts per billion (ppb) (commercially available as the Charm Pesticide Test). To measure the extent of insecticide inhibition of the enzyme, a specific bioluminescent substrate is used. The signal is counter-proportional to the amount of insecticides. Random sampling of four baby food brands and testing for the cumulative levels of organophosphate and N-methylcarbamate insecticides found carbaryl to be the most common residue. Out of the 155 samples tested there were 132 negative samples (85.2%) and 23 suspected positive samples (14.2%). The suspected positive samples were further analyzed by high-performance liquid chromatography (HPLC) and gas chromatography/mass spectrometry (GC/MS). Carbaryl was confirmed in 18 of the samples. One of the samples contained an active metabolite of tetrachlorvinphos and in 3 of the positive samples an insecticide could not be identified by GC/MS. One positive sample was not processed for confirmation due to high fat content
ISSN:0362-028X
1944-9097
DOI:10.4315/0362-028X-59.3.306