A reactive macromolecular matrix for protein immobilization on a gold surface. Application in surface plasmon resonance

A reactive copolymer bearing functions able to react at room temperature with amino groups has been immobilized on a cysteamine-coated gold surface. Polymer immobilization, covalent coupling of rabbit immunoglobulin G and antigen-antibody reactions were studied using a surface plasmon resonance (SPR...

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Bibliographic Details
Published in:Sensors and actuators. B, Chemical Chemical, 1995-10, Vol.29 (1), p.268-273
Main Authors: Millot, M.C., Martin, F., Bousquet, D., Sébille, B., Lévy, Y.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biosensors
Biotechnology
Cysteamine
Fundamental and applied biological sciences. Psychology
Immunosensors
Methods. Procedures. Technologies
Rabbit immunoglobulin G
Reactive polymer
Surface plasmon resonance sensors
Various methods and equipments
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Summary:A reactive copolymer bearing functions able to react at room temperature with amino groups has been immobilized on a cysteamine-coated gold surface. Polymer immobilization, covalent coupling of rabbit immunoglobulin G and antigen-antibody reactions were studied using a surface plasmon resonance (SPR) technique. The effect of a protein A layer on the antigen-antibody reaction was examined.
ISSN:0925-4005
1873-3077
DOI:10.1016/0925-4005(95)01693-7