Expression of a Candidate Cadherin in T Lymphocytes

Cadherins are homotypic adhesion molecules that classically mediate interactions between cells of the same type in solid tissues. In addition, E-cadherin is able to support homotypic adhesion of epidermal Langerhans cells to keratinocytes (Tang, A., Amagai, M., Granger, L. G., Stanley, J. R. & U...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1996-06, Vol.93 (13), p.6567-6571
Main Authors: Cepek, Karyn L., Rimm, David L., Brenner, Michael B.
Format: Article
Language:English
Subjects:
Animals
Antiserum
beta Catenin
Blood
Cadherins
Cadherins - genetics
Catenins
Cell Adhesion Molecules - immunology
Cell lines
Cells
Cellular biology
Cellular immunity
Cytoskeletal Proteins - immunology
Epithelial cells
Humans
Immune Sera
Immunity (Disease)
Molecules
Precipitin Tests
Proteins
Rabbits
T lymphocytes
T-Lymphocytes - metabolism
Trans-Activators
Tumor Cells, Cultured
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Summary:Cadherins are homotypic adhesion molecules that classically mediate interactions between cells of the same type in solid tissues. In addition, E-cadherin is able to support homotypic adhesion of epidermal Langerhans cells to keratinocytes (Tang, A., Amagai, M., Granger, L. G., Stanley, J. R. & Udey, M. C. (1993) Nature (London) 361, 82-85) and heterotypic adhesion of mucosal epithelial cells to E-cadherin-negative intestinal intraepithelial T lymphocytes. Thus, we hypothesized that cadherins may play a wider role in cell-to-cell adhesion events involving T lymphocytes. We searched for a cadherin or cadherins in T lymphocytes with a pan-cadherin antiserum and antisera against α - or β -catenin, molecules known to associate with the cytoplasmic domain of cadherins. The anti-β -catenin antisera coimmunoprecipitated a radiolabeled species in T-lymphocyte lines that had a molecular mass of 129 kDa and was specifically immunoblotted with the pan-cadherin antiserum. Also, the pan-cadherin antiserum directly immunoprecipitated a 120-kDa radiolabeled species from an 125I surface-labeled Jurkat human T-cell leukemic cell line. After V8 protease digestion, the peptide map of this pan-cadherin-immunoprecipitated, 129-kDa species exactly matched that of the 129-kDa species coimmunoprecipitated with the β -catenin antiserum. These results demonstrate that T lymphocytes express a catenin-associated protein that appears to be a member of the cadherin superfamily and may contribute to T cell-mediated immune surveillance.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.93.13.6567