A xanthine oxidase/colloidal gold enzyme electrode for amperometric biosensor applications

An electrode has been prepared based on xanthine oxidase adsorbed to colloidal gold and evaporated onto the surface of glassy carbon. This electrode responds to xanthine or hypoxanthine in the absence of added mediator by electrochemical oxidation of the enzymatic oxidation product, uric acid, at th...

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Bibliographic Details
Published in:Biosensors & bioelectronics 1996, Vol.11 (5), p.493-502
Main Authors: Zhao, Junguo, O'Daly, J.P., Henkens, R.W., Stonehuerner, J., Crumbliss, A.L.
Format: Article
Language:English
Subjects:
bioelectrode
Biological and medical sciences
biosensor
Biosensors
Biotechnology
colloidal gold
enzyme electrode
Fundamental and applied biological sciences. Psychology
hypoxanthine
Methods. Procedures. Technologies
Various methods and equipments
xanthine
xanthine oxidase
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Summary:An electrode has been prepared based on xanthine oxidase adsorbed to colloidal gold and evaporated onto the surface of glassy carbon. This electrode responds to xanthine or hypoxanthine in the absence of added mediator by electrochemical oxidation of the enzymatic oxidation product, uric acid, at the electrode surface. The electrode can also be used in the presence of an electron transfer mediator to detect other substrates for xanthine oxidase such as 4-hydroxypyrimidine.
ISSN:0956-5663
1873-4235
DOI:10.1016/0956-5663(96)86786-8