Fermentation of glycerol to 1,3-propanediol by Klebsiella and Citrobacter strains

Glycerol-fermenting anaerobes were enriched with glycerol at low and high concentrations in order to obtain strains that produce 1,3-propanediol. Six isolates were selected for more detailed characterization; four of them were identified as Citrobacter freundii , one as Klebsiella oxytoca and one as...

Full description

Saved in:
Bibliographic Details
Published in:Applied microbiology and biotechnology 1990-05, Vol.33 (2), p.121-126
Main Authors: Homann, T, Tag, C, Biebl, H, Deckwer, W.D, Schink, B
Format: Article
Language:English
Subjects:
anaerobiosis
Citrobacter
Enterobacter
fermentation
glycerol
Klebsiella
propanediols
trimethylene glycol
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Glycerol-fermenting anaerobes were enriched with glycerol at low and high concentrations in order to obtain strains that produce 1,3-propanediol. Six isolates were selected for more detailed characterization; four of them were identified as Citrobacter freundii , one as Klebsiella oxytoca and one as K. pneumoniae . The Citrobacter strains formed 1,3-propanediol and acetate and almost no by-products, while the Klebsiella strains produced varying amounts of ethanol in addition and accordingly less 1,3-propanediol. Enterobacterial strains of the genera Enterobacter, Klebsiella , and Citrobacter from culture collections showed similar product patterns except for one group which formed limited amounts of ethanol, but no propanediol. Seven strains were grown in pH-controlled batch cultures to determine the parameters necessary to evaluate their capacity for 1,3-propanediol production.
ISSN:0175-7598
1432-0614
DOI:10.1007/bf00176511