Novel localization of a G protein, G sub(z- alpha ), in neurons of brain and retina

Recently, a cDNA coding for a novel G protein alpha -subunit, G sub(z- alpha ), was isolated from a human retinal cDNA library and shown by Northern blot analysis to be expressed at high levels in neural tissues. By light microscopy, G sub(z- alpha ) was localized to the cytoplasm of neurons, with p...

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Bibliographic Details
Published in:The Journal of neuroscience 1990-01, Vol.10 (8), p.2763-2770
Main Authors: Hinton, DR, Blanks, J C, Fong, HKW, Casey, P J, Hildebrandt, E, Simons, MI
Format: Article
Language:English
Subjects:
brain
guanine nucleotide-binding protein
Mammalia
retina
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Summary:Recently, a cDNA coding for a novel G protein alpha -subunit, G sub(z- alpha ), was isolated from a human retinal cDNA library and shown by Northern blot analysis to be expressed at high levels in neural tissues. By light microscopy, G sub(z- alpha ) was localized to the cytoplasm of neurons, with predominant reactivity in ganglion cells of the retina, Purkinje cells of the cerebellum, and most neurons of the hippocampus and cerebral cortex. Reactivity was confined to perikaryon, dendrites, and a very short segment of proximal axons, except for the retinal ganglion cells, in which the axons in the nerve fiber layer showed intense G sub(z- alpha ) immunoreactivity proximal to the lamina cribrosa. The localization of G sub(z- alpha ) and its unique amino acid sequence suggest that it may have a specialized function in neural tissues.
ISSN:0270-6474