DNA oligomers and duplexes containing a covalently attached derivative of tris(2,2'-bipyridine)ruthenium(II): synthesis and characterization by thermodynamic and optical spectroscopic measurements

Oligonucleotides having the base sequence 5'-GCA(C super(*))TCAG-3' and 5'-GCAC(T super(*))CAG-3' were synthesized where C super(*) and T super(*) equal, respectively, a chemically modified cytidine or thymidine base containing a linker arm terminating in a primary amine. The pri...

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Bibliographic Details
Published in:Journal of the American Chemical Society 1989-08, Vol.111 (18), p.7221-7226
Main Authors: Telser, Joshua, Cruickshank, Kenneth A, Schanze, Kirk S, Netzel, Thomas L
Format: Article
Language:English
Subjects:
Carbohydrates. Nucleosides and nucleotides
Chemistry
Exact sciences and technology
Nucleosides, nucleotides and oligonucleotides
Organic chemistry
Preparations and properties
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Summary:Oligonucleotides having the base sequence 5'-GCA(C super(*))TCAG-3' and 5'-GCAC(T super(*))CAG-3' were synthesized where C super(*) and T super(*) equal, respectively, a chemically modified cytidine or thymidine base containing a linker arm terminating in a primary amine. The primary amine of these modified oligomers reacted specifically with the N-hydroxysuccinimide ester of 4-carboxy-4'-methyl-2,2'-bipyridine to form bipyridine-labeled oligomers, and these oligomers reacted with Ru(bpy) sub(2)(H sub(2)O) sub(2) super(2+) to give oligonucleotides with covalently attached derivatives of Ru(bpy) sub(3) super(2+). Oligonucleotides with nonspecifically bound Ru(bpy) sub(2)(H sub(2)O) sub(x) super(2+), where x = 0 or 1, were also formed, but were chromatographically separated from the former product.
ISSN:0002-7863
1520-5126
DOI:10.1021/ja00200a049