Rhizosphere competence of fluorescent Pseudomonas sp. B24 genetically modified to utilise additional ferric siderophores

• Published in: FEMS microbiology ecology 1996, Vol.19 (4), p.215-225
• Main Authors: Moënne-Loccoz, Yvan, McHugh, Brendan, Stephens, Peter M., McConnell, Fiona I., Glennon, Jeremy D., Dowling, David N., O'Gara, Fergal
• Format: Article
• Language: English
• Subjects: Agronomy. Soil science and plant productions; ApPCR; Beta vulgaris; Biochemistry and biology; Biological and medical sciences; biological control agents; Chemical, physicochemical, biochemical and biological properties; colonization; ferric pseudobactin; Fundamental and applied biological sciences. Psychology; genes; genetic engineering; genetically modified microorganisms; iron; Microbiology; pbua gene; Physics, chemistry, biochemistry and biology of agricultural and forest soils; plasmids; Pseudomonas; rhizosphere; Rhizosphere colonisation; Siderophore; siderophores; Soil science
• ISSN: 0168-6496; 1574-6941
• DOI: 10.1016/0168-6496(96)00007-4

The ability to utilise additional siderophores may increase the ecological fitness of biocontrol inoculants of Pseudomonas in the rhizosphere. Plasmid pCUP2 carries a copy of the gene pbuA coding for the membrane receptor of ferric pseudobactin M114. Pseudomonas sp. B24Rif containing pCUP2 can utilise ferric pseudobactin of P. fluorescens M114 in addition to its own siderophore. A larger fraction of the culturable resident fluorescent pseudomonads in the rhizosphere of sugarbeet grown in a low-iron sandy loam soil could supply siderophore-complexed iron to B24Rif(pCUP2) rather than to B24Rif. However, B24Rif and B24Rif(pCUP2) were found at similar population levels in the rhizosphere for 21 days after their inoculation on seeds. A total of 25 of 43 isolates of resident fluorescent Pseudomonas unable to cross-feed iron to B24Rif could cross-feed B24Rif(pCUP2) and they were subdivided into seven different strains by arbitrary-primed PCR fingerprinting. The siderophores produced by 11 of them were typed by HPLC and they were similar to pseudobactin M114. However, the ability to utilise ferric pseudobactin M114 did not improve the ecological fitness of B24Rif in the rhizosphere of sugarbeet although a larger fraction of the culturable resident fluorescent pseudomonads could supply pseudobactin M114-complexed iron to B24Rif(pCUP2) than to B24Rif.